FUNDAMENTALS OF BIOCHEMISTRY-ACCESS
FUNDAMENTALS OF BIOCHEMISTRY-ACCESS
5th Edition
ISBN: 9781119498742
Author: Voet
Publisher: WILEY
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Chapter 2, Problem 18E

(a)

Summary Introduction

To explain: Whether the phosphoric acid or succinic acid is a better buffer at pH 5.

Concept introduction: The pH of a solution is denoted as the negative logarithm of the hydrogen ion concentration of that solution. The pH in a solution decreases as H+ ion concentration increases in the solution. The pH of a neutral aqueous solution is 7, if it exceeds 7, then it is a base and if it below 7 it is an acid. A buffer is the substance that minimizes the changes in the concentration of H+ and OH-.

(b)

Summary Introduction

To explain: Whether the ammonia or piperidine is a better buffer at pH 9.

Concept introduction: The pH of a solution is denoted as the negative logarithm of the hydrogen ion concentration of that solution. The pH in a solution decreases as H+ ion concentration increases in the solution. The pH of a neutral aqueous solution is 7, if it exceeds 7, then it is a base and if it below 7, then it is an acid. A buffer is the substance that minimizes the changes in the concentration of H+ and OH-.

(c)

Summary Introduction

To explain: Whether the HEPES or Tris is a better buffer at pH 5.

Concept introduction: The pH of a solution is denoted as the negative logarithm of the hydrogen ion concentration of that solution. The pH in a solution decreases as H+ ion concentration increases in the solution. The pH of a neutral aqueous solution is 7, if it exceeds 7, then it is a base and if it below 7 it is an acid. A buffer is the substance that minimizes the changes in the concentration of H+ and OH-.

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