(a) Interpretation:
Suppose you have two
The better process for separation: electrophoresis or isoelectric focusing with the required reasons.
Introduction:
Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.
Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.
(b) Interpretation:
The pH that would be chosen for the separation
Introduction:
Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.
Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.
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- BiochemistryBiochemistryISBN:9781319114671Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.Publisher:W. H. FreemanLehninger Principles of BiochemistryBiochemistryISBN:9781464126116Author:David L. Nelson, Michael M. CoxPublisher:W. H. FreemanFundamentals of Biochemistry: Life at the Molecul...BiochemistryISBN:9781118918401Author:Donald Voet, Judith G. Voet, Charlotte W. PrattPublisher:WILEY
- BiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage LearningBiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage LearningFundamentals of General, Organic, and Biological ...BiochemistryISBN:9780134015187Author:John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. PetersonPublisher:PEARSON
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