Campbell Biology Custom Stony Brook 10 Th Edition
10th Edition
ISBN: 9781269870818
Author: Reece Urry Cain Wasserman Minorsky Jackson
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 20, Problem 14TYU
The water in the Yellowstone National Park hot springs shown here is around 160°F (70°C). Biologists assumed that no species of organismscould live in water above about 130°F(55*C), so thev were surprised to find several species of bacteria there, now called thermophiles
| 15. SYNTHESIZE YOUR KNOWLEDGE |
("heat-lovers"). You've learned in this chapter how an enzyme from one species, Thermus aquaticus, made feasible one of the most important DNA-based techniques used in labs today. ldentify the enzyme, and indicate the value of its being isolated from a thermophile. Suggest other reasons why enzymes from this bacterium (or other therrnophiles) might also be valuable.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Erwin Chargaff did not have any DNA samples from thermoacidophilic bacteria such as those that thrive in the geothermal (hot) springs of Yellowstone Park. Such bacteria had not been isolated in 1951 when Chargaff reported his results. If he had obtained such a sample, what do you think its relative G-C content might have been? Why?
During a hike throughout the deepest depths of the Amazon rainforest, you encounter a chest containing a new enzyme that will revolutionize the modern understanding of life itself. After careful study, it's revealed that this miracle enzyme has the ability to produce a substrate (S) into a product (P), with a K_s of 5 mM. You set up an experiment where the enzyme is added to an initial substrate concentration of 15 μM. The concentration of the substrate is halved after 8 minutes. Using this information, calculate the rate constant k, the vmax, and the product concentration after 14 minutes.
In one final experiment, you add all the necessary components and program the thermocycler to cycle under the following temperatures
Denaturation 95 degrees, 30 seconds, Annealing 65 degrees, 30 seconds, Elongation 68 degrees 30 seconds
You find out that the third time's the charm, but now you have many small incomplete fragments instead. What happened and how did you fix it?
Chapter 20 Solutions
Campbell Biology Custom Stony Brook 10 Th Edition
Ch. 20.1 - Prob. 1CCCh. 20.1 - Prob. 2CCCh. 20.1 - What are some potential difficulties in using...Ch. 20.1 - Prob. 4CCCh. 20.2 - Prob. 1CCCh. 20.2 - Prob. 2CCCh. 20.3 - Based on current knowledge, how would you explain...Ch. 20.3 - Prob. 2CCCh. 20.3 - Prob. 3CCCh. 20.4 - What is the advantage of using stem cells for gene...
Ch. 20.4 - Prob. 2CCCh. 20.4 - Prob. 3CCCh. 20 - Describe how the process of gene doning results in...Ch. 20 - What useful Information is obtained by detecting...Ch. 20 - Describe how, using mice. a researcher could carry...Ch. 20 - What factors affecf whether a given genetic...Ch. 20 - In DNA technology, the term vector can refer to...Ch. 20 - Which of the following tools of DNA technology is...Ch. 20 - Prob. 3TYUCh. 20 - A paleontologist has recovered a bit of tissue...Ch. 20 - Prob. 5TYUCh. 20 - Which of the following is not true of cDNA...Ch. 20 - Expression of a cloned eukaryotic gene in a...Ch. 20 - Which Ii of the following sequences in...Ch. 20 - Prob. 9TYUCh. 20 - Prob. 10TYUCh. 20 - EVOLUTlON CONNECTION Ethical considerations aside,...Ch. 20 - Prob. 12TYUCh. 20 - Prob. 13TYUCh. 20 - The water in the Yellowstone National Park hot...
Additional Science Textbook Solutions
Find more solutions based on key concepts
Propose a model for the assembly of a flagellum in a typical Gram-positive cell envelope.
Prescott's Microbiology
The pedigrees indicated here were obtained with three unrelated families whose members express the same disease...
Genetics: From Genes to Genomes
Match the people in column A to their contribution toward the advancement of microbiology, in column B. Column ...
Microbiology: An Introduction
Jellyfish Lake, located on the Pacific island of Palau, is home to millions of jellyfish. Many years ago, sea l...
BIOLOGY:THE ESSENTIALS (LL) W/CONNECT
Why is it necessary to be in a pressurized cabin when flying at 30,000 feet?
Anatomy & Physiology
Some species of bacteria that live at the surface of sediment on the bottom of lakes are capable of using eithe...
Biology: Life on Earth with Physiology (11th Edition)
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Pollutants such as halogenated aromatic hydrocarbons (HAHs)are usually spotty in their distributions in bodies of water. Thuseven if HAHs are present, fish might be able to avoid beingexposed to them. Suppose you want to determine if the fishliving in an industrialized harbor are in fact more exposed toHAHs than fish in a more pristine harbor. Why might a study ofliver P450 enzymes be particularly useful for your purposes?arrow_forwardThree organisms were tested biochemically for their production of energy, and various compounds were measured in each organism. The amounts found are shown in relative units. Organisms ATP NADH NADPH FADH2 Ethanol 1 20 100 0 0 50 2 350 400 0 100 1 3 500 410 200 100 1 Based on this information, which organism is a bacterium, a plant, or an animal?arrow_forwardKnowing that for a bacterial colony to be able to grow it must produce product "3" AND "4", use the information in the image to describe which enzyme(s) are that are Non-Functional in Colony C? Please note error in enzyme description at bottom of image. X converts 1 into 2; Y converts 1 into 3; and z converts 2 into 4.arrow_forward
- While bacteria can meet their nutrient needs and survive on acetic acid as a sole carbon source, human beings cannot. Provide a plausible explanation for this limitation in human beings.arrow_forwardBeer is produced by yeast fermentation of grain to ethanol. Why must the process of beer production be anaerobic?arrow_forwardFind an enzyme that is used by humans for some industrial or useful process (apart from its original purpose; e.g. food production, textiles, agriculture, clinical diagnosis, medical treatment, biofuel production, material polymerization, etc.). How do we obtain or harvest the enzyme? What reaction(s) does it catalyze, and how is this useful for its industrial purpose? 200 words onlyarrow_forward
- Fill the sentence in with the right keyword: Anabolic reactions Sometimes or Alwasys consume energy.arrow_forwardPicture. 1: Fresh potato +H202 H2O+02 (air bubbles) Questions: 1. Why did you use buffer instead of distilled water to dilute the enzyme and the substrate? 2. What do we mean by enzyme specificity? 3. Name the substrate of peptidase, sucrase and amylase? 4. Discuss the factors affecting the rate of an enzymatic reaction, showing how each one affects the rate? 34arrow_forwardDuring times of low oxygen level, many organisms perform fermentation. Who do these organisms need to perform fermentation, the answer is NOT because there is no oxygen.I told you that.arrow_forward
- When the rate of reaction decreases, what is happening to the enzyme, catalase? Did your actual results match your hypotheses? If not, why? Is the optimum temperature the same for all enzymes? Explain.arrow_forwardA competitive inhibitor is one that ________.arrow_forwardRadioactive isotopes (radioisotopes) of elements are commonly used in biological experiments as tracers to follow and detect molecules of interest. For example, photosynthetic intermediates produced during carbon dioxide conversion to sugars were detected by exposing algae to carbon dioxide containing a radioactive form of carbon. This radioactive carbon could be rapidly detected in molecules produced by the algae during carbon fixation and sugar production. Why can scientists substitute radioisotopes for isotopes commonly found in cells when conducting experiments? Select an Answer O A. Radioisotopes of an element differ only in the number of neutrons, so therefore they behave the same way chemically as the isotopes that occur in cells. B. Compounds containing radioisotopes are not acted upon by enzymes, so the radioisotopes will therefore not interfere with normal cellular processes. O C. Radioisotopes contain additional protons, so they are heavier than the nonradioactive isotopes…arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
Chapter 5 Microbial Metabolism; Author: Heather Davis;https://www.youtube.com/watch?v=wH_HrsfDWZw;License: Standard Youtube License