Campbell Biology Plus Masteringbiology
10th Edition
ISBN: 9780321775849
Author: Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson
Publisher: PEARSON
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Chapter 20, Problem 20.2CR
What useful Information is obtained by detecting expression ot specific genes?
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Chapter 20 Solutions
Campbell Biology Plus Masteringbiology
Ch. 20.1 - Prob. 1CCCh. 20.1 - Prob. 2CCCh. 20.1 - What are some potential difficulties in using...Ch. 20.1 - Prob. 4CCCh. 20.2 - Prob. 1CCCh. 20.2 - Prob. 2CCCh. 20.3 - Based on current knowledge, how would you explain...Ch. 20.3 - Prob. 2CCCh. 20.3 - Prob. 3CCCh. 20.4 - What is the advantage of using stem cells for gene...
Ch. 20.4 - Prob. 2CCCh. 20.4 - Prob. 3CCCh. 20 - Describe how the process of gene doning results in...Ch. 20 - What useful Information is obtained by detecting...Ch. 20 - Describe how, using mice. a researcher could carry...Ch. 20 - What factors affecf whether a given genetic...Ch. 20 - In DNA technology, the term vector can refer to...Ch. 20 - Which of the following tools of DNA technology is...Ch. 20 - Prob. 3TYUCh. 20 - A paleontologist has recovered a bit of tissue...Ch. 20 - Prob. 5TYUCh. 20 - Which of the following is not true of cDNA...Ch. 20 - Expression of a cloned eukaryotic gene in a...Ch. 20 - Which Ii of the following sequences in...Ch. 20 - Prob. 9TYUCh. 20 - Prob. 10TYUCh. 20 - EVOLUTlON CONNECTION Ethical considerations aside,...Ch. 20 - Prob. 12TYUCh. 20 - Prob. 13TYUCh. 20 - The water in the Yellowstone National Park hot...
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- Microarrays can be used to determine relative levels of gene expression. In one type of microarray, hybridization of red (experimental) and green (control) labeled cDNAs is proportional to the relative amounts of mRNA in the samples. Red indicates the overexpression of a gene and green indicates the underexpression of a gene in the experimental cells relative to the control cells, yellow indicates equal expression in experimental and control cells, and no color indicates no expression in either experimental or control cells.arrow_forwardMicroarrays can be used to determine relative levels of gene expression. In one type of microarray, hybridization of red (experimental) and green (control) labeled cDNAs is proportional to the relative amounts of mRNA in the samples. Red indicates the overexpression of a gene and green indicates the underexpression of a gene in the experimental cells relative to the control cells, yellow indicates equal expression in experimental and control cells, and no color indicates no expression in either experimental or control cells.In one experiment, mRNA from a strain of antibiotic-resistant bacteria (experimental cells) is converted into cDNA and labeled with red fluorescent nucleotides; mRNA from a nonresistant strain of the same bacteria (control cells) is converted into cDNA and labeled with green fluorescent nucleotides. The cDNAs from the resistant and nonresistant cells are mixed and hybridized to a chip containing spots of DNA from genes 1 through 25. The results are shown in the…arrow_forwardConcentration (%) A researcher is studying environmental conditions that cause the expression of genes that ultimately affects the phenotype. The following schematic diagram depicts the researcher's findings regarding the expression of gene Z (4 points): Regulatory gene DNA lacl MRNA 5 Promoter Operator GeneZ RNA polymerase Protein Active repressor In his research, the scientist determined that the active repressor expression was determined by environmental pH levels. In his investigation, he obtained the following data: Concentration of active repressor at specific pH 12 10 8 16 4 2 12 Concentration (%) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 pH According to the data obtained by the researcher, predict which of the following environmental conditions is mostly likely to cause the expression of the gene Z? Strong acid (pH 2) Neutral (pH 7) Mild base (pH 8) ○ Strong base (pH 12)arrow_forward
- You are interested in finding out in which organ GTF2H5 is highly expressed experimentally. Describe a method in recombinant DNA technology to measure gene expression, explain your rationale, and step by step, what are needed for this method to workarrow_forwardExplain selectable and non selectable markers for the detection of transformants?arrow_forwardWhy are some genes expressed and some not? Please be as detailed as possible.arrow_forward
- What mechanisms are in place to ensure that genetic informationis accurately expressed?arrow_forwardIf a fluorescent spot appears on a microarray, whatinformation does this provide regarding gene expression?arrow_forwardyou are provided with a protein "X" having an expression rate of 10 mg/dl, how you can enhance the expression of that protein up to 50 mg/dl, Using different techniques.arrow_forward
- Can expression or the timing of expression of therapeuticgenes be controlled in a patient so that genes can beturned on or off at a particular time or as necessary?arrow_forwardTwo methods for functionally inactivating a gene without altering the gene sequence involve dominant negative alleles and RNA interference (RNAi). Describe how each method can inhibit expression of a gene.arrow_forwardDoes genetic analysis by ASO testing allow for detection of epigenetic changes that may contribute to a genetic disorder? Explain your answer.arrow_forward
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