Campbell Biology (Custom) (Looseleaf)
10th Edition
ISBN: 9781269755887
Author: Reece
Publisher: PEARSON
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Chapter 20, Problem 8TYU
Which Ii of the following sequences in double-stranded DNA is most likely to be recognized as a cutting site for a restriction enzyme?
(A) AAGG
TTCC
(B) GGCC CCGG
(C) ACCA TGGT
(D) AAAA TTTT
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The restriction enzyme Alu I cleaves at the sequence 5’-AGCT- 3' , and Not I cleaves at 5' -GCGGCCGC-3'. What would be the average distance between cleavage sites for each enzyme on digestion of double-stranded DNA? Assume that the DNA contains equal proportions of A, G, C, and T.
When circular DNA is sequenced, the nucleotide base pairs are numbered starting from a fixed position on the DNA, all the way around, usually in a clockwise manner. a DNA molecule that is 3133 base pairs long is digested with RsaI restriction enzyme recognition sites at base numbers 366, 1534, and 2207. What are the sizes of the DNA fragments that will be produced after the DNA is digested with RsaI?
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Chapter 20 Solutions
Campbell Biology (Custom) (Looseleaf)
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- When linear DNA is sequenced, the nucleotide base pairs are numbered from the start to finish. a DNA molecule that is 3133 base pairs long is digested with RsaI restriction enzyme recognition sites at base numbers 366, 1534, and 2207. What are the sizes of the DNA fragments that will be produced after the DNA is digested with RsaI?arrow_forwardA lagging strand is sketched below. The Okazaki fragment DNA is red, and the RNA primers are dashed orange lines. a) Which Okazaki fragment was made first, A, B, or C? b) When ligase joins fragments A and B, will it act at arrow 1, 2, or both?arrow_forwardConsider the ends of the DNA fragments shown below. They have been produced by digestion of a single sequence of DNA using a number of restriction endonucleases. 1. 5'A 3' 3'TTCGA5' 2. 5'G 3' 3'CAGCT5' 3. 5'AATTC3' 3' G5 4. 5'TCGAC3' 3' G5' 5. 5'GGG 3' 3'CCC 5' Which of these ends are capable of annealing and being joined by DNA ligase?arrow_forward
- You desperately need to join two DNA fragments, one with an EcoRl site and the other with a BamHI site.Both enzymes leave 5' overhangs cutting after the first base. EcoR/ cuts at gaattc, BamHI at ggatcc. Whichof the following statements is true? A. You can not ligate these fragments.B. You can remove the overhangs with another restriction and ligate the blunted ends.C. You can fill in the ends with DNA polymerase and ligate the blunt ends.D. This can be done directly as the 5' overhangs anneal.E. You are better to replace EcoR/ with a related enzyme that leaves a ligatable 3' overhang. Please explain why it's Carrow_forwardRestriction sites are palindromic; that is, they read the same in the5' to 3' direction on each strand of DNA. What is the advantage ofhaving restriction sites organized this way?arrow_forwardHow would the results of this activity have been different if the DNA sequence you digested were circular instead of linear? When restriction digests are performed and analyzed in the laboratory, one lane of the agarose gel is loaded with DNA ladders, which are DNA fragments of known sizes. What is the point of including these size standards?arrow_forward
- A DNA probe with sequence TCAGGCTTCAG would bind most strongly to which of the following DNA fragments? a. AGTCCGAAGTC c. GACTTCGGACT b. TCAGGCTTCAG d. UGAGGCUUGAGarrow_forwardConsider a partial restriction digestion, in which genomic DNA is exposed to a small, limiting amount ofa restriction enzyme for a very short period of time.a. Would the resultant fragments be longer or shorteror the same size as those produced by a completedigestion?b. If you prepared genomic DNA from a tissue sample containing millions of cells, would the fragments produced by partial digestion of DNA fromall of these cells be the same or different?arrow_forwardA lagging strand is sketched below. The Okazaki fragment DNA is red, and the RNA primers aredashed orange lines. Which Okazaki fragment was made first? When ligase joins fragments Aand B, will it act at arrow 1, arrow 2, or both?arrow_forward
- With regard to the experiment described in Figure, The DNA extract was treated with DNase, RNase, or protease.Why was this done? (In other words, what were the researcherstrying to determine?)arrow_forwardYou have discovered an enzyme secreted by a particularly virulent bacterium that cleaves the C2′—C3′ bond in the deoxyribose residues of duplex DNA. What is the eff ect of this enzyme on supercoiled DNA?arrow_forwardA molecule of double-stranded DNA that is 5 million base pairs long has a base composition that is 62% G + C. How many times, on average, are restriction sites for the following restriction enzymes likely to be present in this DNA molecule? a. HindIII (recognition sequence is AAGCTT)arrow_forward
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