Campbell Biology (Custom) (Looseleaf)
Campbell Biology (Custom) (Looseleaf)
10th Edition
ISBN: 9781269755887
Author: Reece
Publisher: PEARSON
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Chapter 20, Problem 14TYU

The water in the Yellowstone National Park hot springs shown here is around 160°F (70°C). Biologists assumed that no species of organismscould live in water above about 130°F(55*C), so thev were surprised to find several species of bacteria there, now called thermophiles

15. SYNTHESIZE YOUR KNOWLEDGE

Chapter 20, Problem 14TYU, The water in the Yellowstone National Park hot springs shown here is around 160F (70C). Biologists

("heat-lovers"). You've learned in this chapter how an enzyme from one species, Thermus aquaticus, made feasible one of the most important DNA-based techniques used in labs today. ldentify the enzyme, and indicate the value of its being isolated from a thermophile. Suggest other reasons why enzymes from this bacterium (or other therrnophiles) might also be valuable.

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In one final experiment, you add all the necessary components and program the thermocycler to cycle under the following temperatures Denaturation 95 degrees, 30 seconds, Annealing 65 degrees, 30 seconds, Elongation 68 degrees 30 seconds  You find out that the third time's the charm, but now you have many small incomplete fragments instead. What happened and how did you fix it?
Based on our current knowledge of biology on Earth, above what temperature (in °C) would biological activity become impossible, and why? Based on our current knowledge of extremophiles, below what temperature (in °C) would the last surviving organisms cease their activity?
Please answer clearly and directlyAn enzyme that works best at pH 2 and temperature 35-39°C was dissolved in water then boiled. Afterwards, the substrate was added. After a while, no reaction occurred and the substrate wasn't converted to products. Explain why the result was negative assuming that the enzyme is correct for the substrate
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