Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 20.3, Problem 2COMQ
Summary Introduction
Introduction:
Transposition is a process in which jumping genes move from the one segment of a chromosome to another or on the same chromosome. hatTransposase belongs to the RNase (ribonuclease) family of proteins, which also includes the retroviral integrases.
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Transposase protein cana. bind to DNA.b. catalyze the excision of a transposable element froma donor site.c. catalyze the insertion of a transposable element intoa target site.d. All of the above
What is Sanger sequencing? Why do we use ddNTP? How to read a DNA sequence gel? c. What is a cDNA seq (RNA seq)? d. What is the main difference between a genomic and a transcriptome study?
Part A:
During cloning, the DNA is cut with a restriction enzyme giving it what?
A.
more introns
B.
sticky ends
C.
a polyA tail
D.
a binding site for a transcription factor
Part B:
If the scientist wanted to have a large number of copies of the gene for use in further study, which technique would the scientist use?
A.
PCR
B.
epigenetics
C.
hybridization
D.
gel electrophoresis
Part C:
If this gene were found to be expressed at different levels in different cells of the same organism, what would be responsible?
A.
transcription factor
B.
rRNA
C.
ribosome
D.
RNA polymerase
Part D:
If a disease were identified as being caused by defects in the cytochrome c gene, then the copy isolated could be used for what?
A.
PCR
B.
gene therapy
C.
stem cell work
D.
DNA fingerprinting
Chapter 20 Solutions
Genetics: Analysis and Principles
Ch. 20.1 - 1. Homologous recombination refers to the exchange...Ch. 20.1 - During the molecular process of homologous...Ch. 20.1 - 3. A key difference between the original Holliday...Ch. 20.1 - Which of the following mechanisms can cause gene...Ch. 20.2 - 1. During site-specific recombination that occurs...Ch. 20.2 - Prob. 2COMQCh. 20.3 - Which of the following types of transposable...Ch. 20.3 - Prob. 2COMQCh. 20.3 - Prob. 3COMQCh. 20 - 1. Describe the similarities and differences...
Ch. 20 - Prob. 2CONQCh. 20 - 3. Which steps in the double-strand break model...Ch. 20 - Prob. 4CONQCh. 20 - Prob. 5CONQCh. 20 - Prob. 6CONQCh. 20 - Prob. 7CONQCh. 20 - 8. What is gene conversion?
Ch. 20 - Make a list of the differences between the...Ch. 20 - Prob. 10CONQCh. 20 - Prob. 11CONQCh. 20 - 12. According to the double-strand break model,...Ch. 20 - What type of DNA structure is recognized by RecG...Ch. 20 - Briefly describe three ways that antibody...Ch. 20 - 15. Describe the functions of the RAG1 and RAG2...Ch. 20 - According to the scenario shown in Figure 20.7,...Ch. 20 - Prob. 17CONQCh. 20 - Prob. 18CONQCh. 20 - 19. Why does transposition always produce direct...Ch. 20 - 20. Which types of TEs have the greatest potential...Ch. 20 - Prob. 21CONQCh. 20 - 22. Let’s suppose that a species of mosquito has...Ch. 20 - This chapter describes different types of TEs,...Ch. 20 - Prob. 24CONQCh. 20 - Prob. 25CONQCh. 20 - 26. What is the difference between an autonomous...Ch. 20 - 1. Briefly explain how McClintock determined that...Ch. 20 - The work of McClintock showed that the presence of...Ch. 20 - 3. In your own words, explain the term transposon...Ch. 20 - Prob. 4EQCh. 20 - 5. Gerald Rubin and Allan Spradling devised a...Ch. 20 - Make a list of the similarities and differences...Ch. 20 - Prob. 2QSDCCh. 20 - Prob. 3QSDC
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Describe What are the sticky ends of the restriction fragments? Select one: a. The surfaces of sticky ends contain matching base pairs, allowing fragments to splice. b. The surfaces of sticky ends have glue like substance that allow fragments to splice. c. The surfaces of sticky ends contain the exact same nucleotides, allowing fragments to bond. d. The surfaces of sticky ends have velcro like structure, allowing fragments to bond.arrow_forwardTautomerization: A. Causes frameshift insertions B. Causes frameshift deletions C. Results in permanent chemical changes in nitrogenous bases D. Cause transition mutations E. Cause transversion mutationsarrow_forwardWhich of the following is true of reverse transcriptase?a. It is required for the movement of DNA transposons.b. It catalyzes the synthesis of DNA from RNA.c. It is required for the transposition of retrotransposons.d. b and c are correct.arrow_forward
- In gene silencing, the “dicer” enzymea. assembles siRNAs into a RISC complex.b. unwinds the RISC complex.c. binds siRNA to mRNA sequences complementary to it.d. slices up double-stranded RNA moleculesarrow_forwardWhich of the following is a function of a restriction endonuclease? a. It cleaves a DNA molecule at a specific site. b. It cleaves a DNA molecule at random locations. c. It removes specific codons from an mRNA molecule. d. It removes specific exons from an mRNA molecule.arrow_forwardIf you want to express a eukaryotic gene in a bacterial cell, you ned to (select all that apply): a.) add a bacterial promoter in front of the gene b.) remove introns from the gene c.) add a polyA tail sequence d.) add eukaryotic tRNAsarrow_forward
- the most efficient general strategy for whole genome sequencing is ? (a) double the coding sequence after sequencing the proteins (b) shotgun sequence and assemble based on overlaps (c) identify mutations that affect glycolysis (d) obtain recombinant DNA clone maps before starting the sequencing (e) obtain comprehensive SNP maps before determining the order of DNA clonearrow_forwardThe function of transposase isa. to recognize inverted repeats.b. to remove a TE from its original site.c. to insert a TE into a new site.d. all of the above.arrow_forwardThe restriction endonucleases used in recombinant DNA work: a. are synthesized by bacteria b. recognize sequences 14-16 bp long c. cut the DNA outside the recognition sequence d. all the above are truearrow_forward
- Why can the transcriptome not be used to predict the proteome with complete accuracy? a. It cannot be sequenced like the genome can be. b. The transcriptome is too dynamic to be used to make predictions. c. Not all genes are transcribed. d. Many transcripts are alternatively spliced to produce different proteins.arrow_forwardWhat type of mutation causes the "mutant" variety of the PTC gene? a A point mutation which lead to a nonsense mutation b A addition mutation which lead to a frameshift c A substitution mutation which lead to a missense mutation d A deletion mutation which lead to a SNP mutationarrow_forward(a) Why can there be multiple codons for an amino acid? Why would this have evolved? (b) What is the advantage of Illumina Next Generation Sequencing?arrow_forward
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