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Concept explainers
(a) Interpretation:
Identify the parts of the C0t curve that correcspond to anealing of (1) unique sequences, (2) modereately repetetive sequence, and (3) highly repetetive sequences.
Introduction:
The C0t curve is the graphical representation of the percentage of the single stranded DNA as a function of log of cot values. The cot analysis is the representation of DNA renaturation kinetics.
Pictorial representation:
The given below is the C0t analysis, in which the types of DNA sequences are labeled:
(b) Interpretation:
Identify the parts of the C0t curve that correcspond to anealing single copy gene.
Introduction:
The C0t curve is the graphical representation of the percentage of the single stranded DNA as a function of log of cot values. The cot analysis is the representation of DNA renaturation kinetics. The single copy genes are the unique sequences.
Pictorial representation:
The given below is the C0t analysis, in which the types of DNA renaturation obtained is labeled:
(c) Interpretation:
The difference in the annealing activity of the cDNA and normal ovalbumin.
Introduction:
The C0t curve is the graphical representation of the percentage of the single stranded DNA as a function of log of cot values. The cot analysis is the representation of DNA renaturation kinetics.
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Chapter 21 Solutions
Pearson eText for Biochemistry: Concepts and Connections -- Instant Access (Pearson+)
- BiochemistryBiochemistryISBN:9781319114671Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.Publisher:W. H. FreemanLehninger Principles of BiochemistryBiochemistryISBN:9781464126116Author:David L. Nelson, Michael M. CoxPublisher:W. H. FreemanFundamentals of Biochemistry: Life at the Molecul...BiochemistryISBN:9781118918401Author:Donald Voet, Judith G. Voet, Charlotte W. PrattPublisher:WILEY
- BiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage LearningBiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage LearningFundamentals of General, Organic, and Biological ...BiochemistryISBN:9780134015187Author:John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. PetersonPublisher:PEARSON
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