Chapter 21, Problem 3P

Interpretation Introduction

(a) Interpretation:

The other half of a restriction site should be drawn if the one-half is as follows:

Concept Introduction:

The restriction site also known as the restriction sequence is an enzyme that splits DNA into fragments at a recognized site inside a molecule. The restriction site's other part is given and the type II cleavage site with the blunt-ended duplex DNA is shown with heavy arrows. The type II cleavage sites yielding staggered cut is given by light arrows. For the type I restriction endonuclease recognition, the duplex cut is at 1 kilobase away and identical copies of DNA sequence interval is 4096 kilobase pairs as the sequence is 6 base longs with 4 possibility positioned nucleotides.

Interpretation Introduction

(b) Interpretation:

Type II cleavage sites producing blunt-ended duplex DNA should be identified using heavy arrows.

Concept Introduction:

The restriction site also known as the restriction sequence is an enzyme that splits DNA into fragments at a recognized site inside a molecule. The restriction site's other part is given and the type II cleavage site with the blunt-ended duplex DNA is shown with heavy arrows. The type II cleavage sites yielding staggered cut is given by light arrows. For the type I restriction endonuclease recognition, the duplex cut is at 1 kilobase away and identical copies of DNA sequence interval is 4096 kilobase pairs as the sequence is 6 base longs with 4 possibility positioned nucleotides.

Interpretation Introduction

(c) Interpretation:

Type II cleavage sites producing staggered cuts converted to recombinant DNA molecules by DNA ligase without enzyme involvement should be identified using light arrows.

Concept Introduction:

The restriction site also known as the restriction sequence is an enzyme that splits DNA into fragments at a recognized site inside a molecule. The restriction site's other part is given and the type II cleavage site with the blunt-ended duplex DNA is shown with heavy arrows. The type II cleavage sites yielding staggered cut is given by light arrows. For the type I restriction endonuclease recognition, the duplex cut is at 1 kilobase away and identical copies of DNA sequence interval is 4096 kilobase pairs as the sequence is 6 base longs with 4 possibility positioned nucleotides.

Interpretation Introduction

(d) Interpretation:

The place where the duplex be cut when the recognition site for type I restriction endonuclease should be identified.

Concept Introduction:

The restriction site also known as the restriction sequence is an enzyme that splits DNA into fragments at a recognized site inside a molecule. The restriction site's other part is given and the type II cleavage site with the blunt-ended duplex DNA is shown with heavy arrows. The type II cleavage sites yielding staggered cut is given by light arrows. For the type I restriction endonuclease recognition, the duplex cut is at 1 kilobase away and identical copies of DNA sequence interval is 4096 kilobase pairs as the sequence is 6 base longs with 4 possibility positioned nucleotides.

Interpretation Introduction

(e) Interpretation:

The average interval of identical copies of the given sequence in DNA which is random should be identified in kilobase pairs.