BIOLOGY (LL)
5th Edition
ISBN: 9781264115495
Author: BROOKER
Publisher: MCG
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Chapter 21.1, Problem 1CS
Summary Introduction
To explain: The general properties of the plasmid.
Introduction: Genome is defined as the complete genetic material that is present in an organism. It consists of both the coding as well as the non-coding parts of DNA. The study of the genome is known as genomics. Genomics contains an important technique by which multiple and exact copies of a gene can be produced. This technique is known as gene cloning.
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Objective: Get a sense of how genomics, the study of the genome in its entirety,needs to think about how to go about its research.
Geonomic DNA is broken up into fragments. The 5’ and 3’ ends of each fragment(a “read”) are sequenced. The sequenced reads are assembled together intocontiguous sequences (“contigs”) based on sequence similarity.
The idea is to sequence enough random fragments so that every nucleotide in thegenome is represented on some read. The number of such fragments needed iscalled the coverage, c.
The coverage c can be calculated by the formula RL/G, where R is the number ofreads sequenced, L is the average length of a read and G is the total length of thegenome. The units of length are bases (b) or base pairs (bp).
Consider a genome whose length is 1000 bp. “Shotgun” sequencing techniquesare applied to the genome, resulting in 20 reads, with an average length of 50 bp.A very important point is that, even though 20 x 50 = 1000, there is no guaranteethat ALL…
Objective: Get a sense of how genomics, the study of the genome in its entirety,needs to think about how to go about its research.
Geonomic DNA is broken up into fragments. The 5’ and 3’ ends of each fragment(a “read”) are sequenced. The sequenced reads are assembled together intocontiguous sequences (“contigs”) based on sequence similarity.
The idea is to sequence enough random fragments so that every nucleotide in thegenome is represented on some read. The number of such fragments needed iscalled the coverage, c.
The coverage c can be calculated by the formula RL/G, where R is the number ofreads sequenced, L is the average length of a read and G is the total length of thegenome. The units of length are bases (b) or base pairs (bp).
Consider a genome whose length is 1000 bp. “Shotgun” sequencing techniquesare applied to the genome, resulting in 20 reads, with an average length of 50 bp.A very important point is that, even though 20 x 50 = 1000, there is no guaranteethat ALL…
2- Which statement is false is regarding GEMC ?
(a)typical cloning vectors involbe plasmids and viruses
(b)eukaryotic genes may only be introduced and expressed in eukaryotic microbes such as yeasts
(c)horizontal gene transfer methods may be manipulated to introduce new genes
(d) expressions of introduced genes can be monitored through the use of marker genes.
(e)it is possible for multiple genes may be added to microbes from other sources .
asap please
Chapter 21 Solutions
BIOLOGY (LL)
Ch. 21.1 - Prob. 1CSCh. 21.1 - Prob. 1CCCh. 21.1 - Prob. 2CCCh. 21.1 - Prob. 3CCCh. 21.2 - Prob. 1CCCh. 21.2 - Prob. 2CCCh. 21.2 - Prob. 3CCCh. 21.3 - Prob. 1EQCh. 21.3 - Prob. 2EQCh. 21.3 - Prob. 3EQ
Ch. 21.4 - Prob. 1CCCh. 21.4 - Prob. 1CSCh. 21.5 - Prob. 1CSCh. 21.5 - Repetitive Sequences and Transposable Elements...Ch. 21 - Prob. 1TYCh. 21 - DNA ligase is needed in a cloning experiment a. to...Ch. 21 - Prob. 3TYCh. 21 - Why is Taq polymerase used in PCR rather than...Ch. 21 - Lets suppose you want to clone a gene that has...Ch. 21 - In the CRISPR-Cas technology for editing genes,...Ch. 21 - Prob. 7TYCh. 21 - The enzyme that helps short segments of DNA move...Ch. 21 - Prob. 9TYCh. 21 - Which of the following was not a goal of the Human...Ch. 21 - Prob. 1CQCh. 21 - Briefly describe whether or not each of the...Ch. 21 - Prob. 3CQCh. 21 - Identify and discuss three important advances that...Ch. 21 - Prob. 2COQ
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- Pls help ASAParrow_forwardWhat is an engineered plasmid used for? What are the important features of\ engineered plasmids?arrow_forwardNeed bioinformatics help. 1) Examine the quality of the raw sequencing data 2) Trim low quality sequences and sequencing adapters from the raw reads 3) Align trimmed reads against the reference genome 4) Count number of reads mapped per annotated gene 5) Differential expression analysis 6) Visualize sequencing reads aligned to the reference genome 7) Gene set enrichment analysis (GSEA) Choices: Google Colab UCSC Genome Browser fasterq-dump MultiQC BLAST DESeq2 STAR Gorilla featureCounts SNPEff samtools flagstat BWA mem Integrative Genome Viewer (IGV) LoFreq Fastp FastQC align.seq 2) Trim low quality sequences and sequencing adapters from the raw reads 3) Align trimmed reads against the reference genome 4) Count number of reads mapped per annotated gene 5) Differential expression analysis 6) Visualize sequencing reads aligned to the reference genome 7) Gene set enrichment analysis (GSEA) Choices: Google Colab UCSC Genome Browser…arrow_forward
- How can site-specific recombination be used in recombinant DNA technology? Explain with an examplearrow_forwardQ. Based on your understanding of plasmids’ answer the following questions.1. Why is a daughter cell that fails to inherit a kind of plasmid dies? 2. How transfer of Col E1 plasmid to an F cell occurs? 3. What component you will must include in constructing a plasmid cloning vector that can replicate in both bacteria and yeast? 4. How copy number of a plasmid may affect its segregation? 5. How the yeast 2u circle maintains 80 copies in a cell?arrow_forward@saparrow_forward
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