Concept explainers
(a)
Interpretation:
The order of elution of aspartate before serine by
Concept introduction:
Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.
Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.
Cation-exchange chromatography separate amino acids according to their
(b)
Interpretation:
The order of elution of serine before alanine by
Concept introduction:
Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.
Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.
Cation-exchange chromatography separate amino acids according to their
(c)
Interpretation:
The order of elution of valine before leucine by
Concept introduction:
Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.
Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.
Cation-exchange chromatography separate amino acids according to their
(d)
Interpretation:
The order of elution of tyrosine before phenylalanine by
Concept introduction:
Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.
Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.
Cation-exchange chromatography separate amino acids according to their
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CHEM 262 ORG CHEM EBOOK DIGITAL DELIVERY
- d. réčombinants 20. What functional group is found on every amino acid not in a zwitterion form? a. -SH b. -NH, C. -C- d. -S-S- e. no correct response is given 21. The peptide linkage that bonds the monomers together in proteins is also corrarrow_forwardExplain the order of elution (with a buffer of pH 4) of the following pairs of amino acids through a column packed with Dowex 50 a. aspartate before serine c. valine before leucine b. serine before alanine d. tyrosine before phenylalaninearrow_forwardCould someone explain what this is saying? I would like a better understanding. FIGURE D CPP32 (Caspase-3) protease activity. Aliquots of cell lysates (50μL) were incubated with an equal volume (50 μL) of the reactionbuffer and 5 μL of 7-amino-4-trifluoromethyl coumarin (AFCDEVD) for 1 hour at 37˚C (Clontech). The shift in fluorescenceemission of AFC-DEVD, on its proteolysis to free AFC by theprotease, was detected in a fluorometer, using a 400 nm excitationfilter and 505 nm emission filter.arrow_forward
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