Brock Biology of Microorganisms, Books a la Carte Plus Mastering Microbiology with Pearson eText -- Access Card Package (15th Edition)
15th Edition
ISBN: 9780134603940
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Chapter 21.7, Problem 1CR
Summary Introduction
Higher concentration of Mercury ion (Hg2+) and Methylmercury (CH3Hg+) are very toxic to both macroorganisms and microorganisms. Many gram-positive and gram-negative bacteria can convert these toxic forms to relatively less toxic or non-toxic forms. Mercury resistant bacteria use enzyme organomercury lyase to degrade toxic form such as CH3Hg+ to Hg2+ and methane. These bacteria also employ the use of enzyme mercuric reductase to reduce Hg2+ to Hg0.
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1)Explain why there is a part of the Lacz gene in pUC18119 and also how the natural biochemical process wherein it normally functions is used during cloning by molecular biology.
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Chapter 21 Solutions
Brock Biology of Microorganisms, Books a la Carte Plus Mastering Microbiology with Pearson eText -- Access Card Package (15th Edition)
Ch. 21.1 - How is new organic matter made in nature?Ch. 21.1 - In what ways are oxygenic photosynthesis and...Ch. 21.1 - What is a methane hydrate?Ch. 21.1 - The oxygen and carbon cycles are interconnected...Ch. 21.2 - Why does Syntrophomonas need a partner organism in...Ch. 21.2 - Prob. 2MQCh. 21.2 - What is the final product of acetogenesis?Ch. 21.2 - Prob. 1CRCh. 21.3 - What is nitrogen fixation and why is it important...Ch. 21.3 - Prob. 2MQ
Ch. 21.3 - How does the compound nitrapyrin benefit both...Ch. 21.3 - The principal form of nitrogen on Earth is N2,...Ch. 21.4 - Is H2S a substrate or a product of the...Ch. 21.4 - Prob. 2MQCh. 21.4 - Prob. 3MQCh. 21.4 - Bacteria play major roles in both the oxidative...Ch. 21.5 - In what oxidation state is Fe in Fe(OH)3? In FeS?...Ch. 21.5 - Prob. 2MQCh. 21.5 - Why is excreted organic matter important to many...Ch. 21.5 - Prob. 1CRCh. 21.6 - How does the formation of CaCO3 skeletons by...Ch. 21.6 - Prob. 2MQCh. 21.6 - Prob. 1CRCh. 21.7 - What forms of mercury are most toxic to organisms?Ch. 21.7 - Prob. 2MQCh. 21.7 - Prob. 3MQCh. 21.7 - Prob. 1CRCh. 21.8 - What is the greenhouse effect and what causes it?Ch. 21.8 - What is the fate of most nitrogen used in...Ch. 21.8 - Why are the OMZs expanding and what are the likely...Ch. 21.8 - Prob. 1CRCh. 21 - Compare and contrast the carbon, sulfur, and...Ch. 21 - 14C-labeled cellulose is added to a vial...Ch. 21 - Prob. 3AQ
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- 1.Your goal is to design a strategy to create thisfinal pDHFR plasmid for fusion protein expressionfrom the materials available:You have an empty pET21a expression vector and another vectorthat contains the fusion protein nucleotide sequence (pBluescript + GST-DHFR-His). (#) indicatesbp location ofenzyme cut: a.EcoRIonly, HindIII only, NotIonly, EcoRI and HindIII, EcoRI and NotI, or HindIII and NotI? b.Briefly explain the rationale for your selection. Please connect your rationale to the need for compatible ends without any further manipulation and required directionality of the DHFR fusion protein coding sequence relative to the T7 promoter that will drive its expression. c.What is the final size(kb)of your desired pDHFR plasmid ligation product based onyour design? d. Complete the chart providedto indicate thesize of all fragments(in bp)resultingfrom complete digestion of each plasmid givenyour strategy, andthe fragment(in kb)from each you would isolate for ligation.Please mind…arrow_forwardA plasmid that is both ampicillin and tetracyclineresistant is cleaved with PstI, which cleaves within theampicillin resistance gene. The cut plasmid is ligated withPstI-digested Drosophila DNA to prepare a genomic library,and the mixture is used to transform E. coli K12. Question: If recombinant cells were plated on medium containingampicillin or tetracycline and medium withboth antibiotics, on which plates would you expectto see growth of bacteria containing plasmids withDrosophila DNA inserts?arrow_forwardE. coli strain BW25113 can grow on (D) Arg as the sole carbon source. A scientist working with you on this project has discovered that mice encode a protease that cancleave between (D)Arg‐(D)Arg. a. In a set of discrete steps outline how to construct this genomic library so that recombinant expression can be performed in E. coli b. Once the genomic library has been constructed, describe in DISCRETE steps how you would isolate the DNA sequence that encodes for the protease that cleaves between (D)Ag-(D)Argarrow_forward
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