Concept explainers
Interpretation:
The elution order of given amino acids with
Concept introduction:
Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.
Dowex-1 is an anion-exchange resins with less polar used for anion-exchange chromatography.
Anion-exchange chromatography separate amino acids according to their
Want to see the full answer?
Check out a sample textbook solutionChapter 22 Solutions
Organic Chemistry
- For the three tripeptides TED, RAY and ALI, determine in which order they will be eluted at pH 7.0 from cation exchange chromatography column.arrow_forwardHow does the shape of a titration curve confirm the fact that the pH region of greatest buffering power for an amino acid solution is around its pK's? Name four factors (bonds or other forces) that contribute to stabilizing the native structure of a protein, and describe one condition or reagent that interferes with each type of stabilizing force. Why is silk fibroin so strong, but at the same time so soft and flexible?arrow_forwardExplain the order of elution (with a buffer of pH 4) of the following pairs of amino acids through a column packed with Dowex 50a. aspartate before serine c. valine before leucineb. serine before alanine d. tyrosine before phenylalaninearrow_forward
- Look up the pKa values for trimethylamine, dimethylamine, ethylamine, and ammonia. Predict the order of elution of these compounds from a cation-exchange column eluted with a gradient of increasing pH, beginning at pH 7.arrow_forwardExplain the order of elution (with a buffer of pH 4) of the following pairs of amino acids through a column packed with Dowex 50 a. aspartate before serine c. valine before leucine b. serine before alanine d. tyrosine before phenylalaninearrow_forwardDetermine the buffer capacity of Alanine and Histidine at pH 2.0 and 6.0 respectively and discuss the results.arrow_forward
- 1. A mixture of proteins (pepsinogen, myoglobin, and cytochrome c) is subjected to anion exchange chromatography at pH 5.0. In what order would the following proteins be eluted from the DEAE cellulose anion exchanger by an increasing salt gradient: cytochrome c (pl, 10.6), pepsinogen (pl, 1.0) and myoglobin (pl, 6.8)arrow_forwardAre the number of spots shown on SDS-PAGE electrophoresis dependant on whether the protein is a monomer or tetramer?arrow_forwardAn amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated bypaper chromatography. The solvent is less polar than water. Which of these amino acids will have the highest Rf value and which the lowest? Explain. Treatment of a new protein with dansyl chloride reveals two (2) dansyl-labelled derivatives of amino acids, alanine and methionine. What can you deduce about the structure of the protein from these results? Name the amino acids that contribute atoms to both purine and pyrimidine rings.arrow_forward
- Show the condensation reactions that result in the formation of the tetrapeptide Asp-Gly-Ser- Gln. After you have finished the synthesis change the structure to show how it would exist in a cell at physiological pH (pH = 7).arrow_forwardThe pka of ascorbic acid is 4.2 at 24C. At what pH is the ratio of the deprotonated form to the protonated form 10:1? Prove using the Henderson-Hasselback equation. How to resolve this.arrow_forwardFor the determination of Pb in blood, a 5.00 mL sample was treated with trichloroacetic acid to precipitate the proteins. After centrifugation, the resulting solution was brought to pH 3 and extracted with two 5 mL portions of methyl isobutyl ketone containing the lead complexing agent APCD (ammonium 1-pyrrolidine carbodithiolate). The extract was drawn directly into an air-acetylene flame which produced an absorbance of 0.444 at 283.3 nm. Two 5.00 mL aliquots of solutions containing 0.250 and 0.450 ppm of Pb were subjected to the same sample treatment and the absorbance that occurred was 0.396 and 0.599 respectively. Calculate the concentration of Pb in µg/dL in the blood.arrow_forward
- Organic ChemistryChemistryISBN:9781305580350Author:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. FootePublisher:Cengage LearningPrinciples of Instrumental AnalysisChemistryISBN:9781305577213Author:Douglas A. Skoog, F. James Holler, Stanley R. CrouchPublisher:Cengage Learning