How the study of LDL receptor binding by a human rhinovirus HRV2, provided support for model of LDL particle displacement should be discussed. Concept Introduction: LDL receptor has five domains. N terminal LDL binding domain contains seven cysteine rich repeats referred as R1 to R7. Next epidermal growth factor repeats and ß propeller module can be found. Those segments are followed by O-linked oligosaccharide, a membrane spanning segment and another segment extended towards the cytosol. LDL particles bind with R4 and R5 cysteine repeats. At low pH, the LDL receptor polypeptide folds so that ß propeller domain is associated with R4 and R5 displacing the LDL particle. These two repeats contain two loops connected by 3 disulfide bonds. Second loop of each repeat have Asp and Glu acidic residues and acts as a Ca 2+ binding site.

Question

Suppose that your Ph.D. mentor asks you to compare the kinetic behavior of the two recently discovered peptidase isozymes PepA and PepB. Your mentor's initial studies identified a pentapeptide substrate called Tide5 that appeared to be able to distinguish the kinetic behaviors of PepA and PepB. All they had time to tell you was this: At low Tide5 concentration, the ?o (initial velocity) of PepA was greater than PepB, but at high Tide5 concentration, the reverse was true. Before you start your kinetic assays, you decide it would be wise to ponder this small bit of information. You have no numbers to work with, but does it tell you something about the relative values of ?m and ?max for PepA and PepB? Select the statements that are consistent with the information that you were given.

Answer 1

Question

Chapter 24, Problem 18P

Interpretation Introduction

Interpretation:

How the study of LDL receptor binding by a human rhinovirus HRV2, provided support for model of LDL particle displacement should be discussed.

Concept Introduction:

LDL receptor has five domains. N terminal LDL binding domain contains seven cysteine rich repeats referred as R1 to R7. Next epidermal growth factor repeats and ß propeller module can be found. Those segments are followed by O-linked oligosaccharide, a membrane spanning segment and another segment extended towards the cytosol. LDL particles bind with R4 and R5 cysteine repeats. At low pH, the LDL receptor polypeptide folds so that ß propeller domain is associated with R4 and R5 displacing the LDL particle. These two repeats contain two loops connected by 3 disulfide bonds. Second loop of each repeat have Asp and Glu acidic residues and acts as a Ca²⁺ binding site.

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Suppose that your Ph.D. mentor asks you to compare the kinetic behavior of the two recently discovered peptidase isozymes PepA and PepB. Your mentor's initial studies identified a pentapeptide substrate called Tide5 that appeared to be able to distinguish the kinetic behaviors of PepA and PepB. All they had time to tell you was this: At low Tide5 concentration, the ?o (initial velocity) of PepA was greater than PepB, but at high Tide5 concentration, the reverse was true. Before you start your kinetic assays, you decide it would be wise to ponder this small bit of information. You have no numbers to work with, but does it tell you something about the relative values of ?m and ?max for PepA and PepB? Select the statements that are consistent with the information that you were given.

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