Chapter 24, Problem 3P

Summary Introduction

To calculate: The molecular weight and the contour length of the genome of Mycoplasma genitalium.

Introduction:

Mycoplasma genitalium is simplest bacterium whose complete genome contains a circular DNA molecule with 580,070 bp. This value is determined by the use of restriction enzymes that cut its DNA infrequently. Its genome is the smallest genome of all self-replicating organisms.

Summary Introduction

To determine: The $L{k}_0$ of Mycoplasma chromosome.

Introduction:

In a double stranded DNA, linking number is a topological characteristics, it does not vary whether the DNA is deformed or bent. It causes both DNA strands to remain intact. Linking number can be written as Lk. Linking number for a circular DNA molecule should be intact, without a cut to possess topological characteristics.

Summary Introduction

To determine: The $LK$, If $\sigma =-0.06$.

Introduction:

A quantity which is independent of the length of DNA molecule is termed as super helical density or specific linking number σ. It can define the change in linking number. It is a measurement of the numbers of turns, eliminated than the number of turns present in relaxed DNA.