Chapter 25, Problem 12P

Summary Introduction

To determine: How the colonies of Salmonella typhimurium auxotrophs grew in histidine deficient medium.

Introduction:

Histidine is an essential amino-acid metabolite required for the growth of the strain of Salmonella typhimurium. It is added in the nutrient growth medium during the start of nutrient culture.

Summary Introduction

To determine: The growth of Salmonella typhimurium colonies after addition of 0.4 µg 2-aminoanthracene.

Introduction:

Chemical 2-aminoanthracene contains an aromatic ring which is used for testing reverse mutation in strains of bacteria.

Summary Introduction

To determine: The carcinogenicity of chemical 2-aminoanthracene.

Introduction:

The 2-aminoanthracene is an aromatic cyclic ring chemical which can cause point or frameshift mutations in the genome of the species of bacteria.