BIOCHEMISTRY-ACHIEVE (1 TERM)
9th Edition
ISBN: 9781319402853
Author: BERG
Publisher: MAC HIGHER
expand_more
expand_more
format_list_bulleted
Question
Chapter 25, Problem 44P
Interpretation Introduction
Interpretation:
Cells were incubated with 14C labeled glucose in the second position. The uracil synthesized in the cell is found to have labeled carbon (14C) at the 4th and 6th positions. The pattern for such labeling in uracil should be determined.
Concept introduction:
Purines are heterocyclic organic compounds. Purine is composed of two fused rings, a pyrimidine ring, and an imidazole ring. Adenine and guanine, found in
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
On the trail of carbons. Tissue culture cells were incubated with
glutamine labeled with 15NN in the amide group. Subsequently,
IMP was isolated and found to contain some 15N.N. Which atoms
in IMP were labeled?
More ratios. Through the use of nuclear magnetic resonance
spectroscopy, it is possible to determine the ratio between the
protonated and deprotonated forms of buffers. (a) Suppose the ratio
of [ A- ]A I to [HA] is determined to be 0.1 for a buffer with
pKar6.0.pKa = 6.0. What is the pH? (b) For a different buffer,
91974
suppose the ratio of [ A- ]lA J to [HA] is determined to be 0.1 and
the pHpH is 7.0. In this case, what is the pKapKa of the buffer? (c) For
another buffer with pKa=7.5PKa = 7.5 at pH 8.0pH 8.0, what is the
expected ratio of [ A- ][A ] to [HA]? do
The allosteric site
1). Draw a representation of an allosteric binding site of an enzyme.
must bind an allosteric modifier that maintains three hydrophobic regions, a polar
charged region and two regions of hydrogen bonding. You choose the order or
organization of the characteristics in the allosteric site - label all regions.
Details
the allosteric site is composed of faces of two separate beta sheets and two
separate alpha helices. You might decide that each beta sheet or each alpha helix
contributes one amino acid R group to the allosteric site or you may decide that one of
the beta sheets or one of the alpha helixes contributes two or more amino acid R groups
to the allosteric organization. Your choice but please show R group structure in the
allosteric site!
Chapter 25 Solutions
BIOCHEMISTRY-ACHIEVE (1 TERM)
Ch. 25 - Prob. 1PCh. 25 - Prob. 2PCh. 25 - Prob. 3PCh. 25 - Prob. 4PCh. 25 - Prob. 5PCh. 25 - Prob. 6PCh. 25 - Prob. 7PCh. 25 - Prob. 8PCh. 25 - Prob. 9PCh. 25 - Prob. 10P
Ch. 25 - Prob. 11PCh. 25 - Prob. 12PCh. 25 - Prob. 13PCh. 25 - Prob. 14PCh. 25 - Prob. 15PCh. 25 - Prob. 16PCh. 25 - Prob. 17PCh. 25 - Prob. 18PCh. 25 - Prob. 19PCh. 25 - Prob. 20PCh. 25 - Prob. 21PCh. 25 - Prob. 22PCh. 25 - Prob. 23PCh. 25 - Prob. 24PCh. 25 - Prob. 25PCh. 25 - Prob. 26PCh. 25 - Prob. 27PCh. 25 - Prob. 28PCh. 25 - Prob. 29PCh. 25 - Prob. 30PCh. 25 - Prob. 31PCh. 25 - Prob. 32PCh. 25 - Prob. 33PCh. 25 - Prob. 34PCh. 25 - Prob. 35PCh. 25 - Prob. 36PCh. 25 - Prob. 37PCh. 25 - Prob. 38PCh. 25 - Prob. 39PCh. 25 - Prob. 40PCh. 25 - Prob. 41PCh. 25 - Prob. 42PCh. 25 - Prob. 43PCh. 25 - Prob. 44PCh. 25 - Prob. 45PCh. 25 - Prob. 46PCh. 25 - Prob. 47PCh. 25 - Prob. 48PCh. 25 - Prob. 49PCh. 25 - Prob. 50PCh. 25 - Prob. 51P
Knowledge Booster
Similar questions
- Ionization State of Histidine.Each ionizable group of an amino acid can exist in one of two states, charged or neutral. The electric charge on the functional group is determined by the relationship between its pKa and the pH of the solution. This relationship is described by the Henderson-Hasselbalch equation. 1.Histidine has three ionizable functional groups. Write the equilibrium equations for its three ion-izationsand assign the proper pKa for each ionization. Draw the structure of histidine in each ionization state.What is the net charge on the histidine molecule in each ionization state? 2.Which structure drawn in (1) corresponds to theionization state of histidine at pH 1, 4, 8, and12?Note that the ionization state can be approximated by treating each ionizable group independently. 3.What is the net charge of histidine at pH 1, 4, 8, and 12? For each pH, will histidine migrate to-ward the anode (+) or cathode (-) when placed in an electric field?arrow_forwardBiological Molecules. How does Mycobacterium tuberculosis obtain the requirements to build its biological macromolecules? discussing where your bacteria gets its basic requirements for the elements of life - carbon, hydrogen, oxygen, nitrogen, phosphorus and sulfur. Basically the nutritional requirements, not specifically each element individually).arrow_forwardPeptide mass determination. You have isolated a proteinfrom the bacterium E. coli and seek to confirm its identityby trypsin digestion and mass spectrometry. Determinationof the masses of several peptide fragments has enabled youto deduce the identity of the protein. However, there is adiscrepancy with one of the peptide fragments, whichyou believe should have the sequence MLNSFK and an(M 1 H)1 value of 739.38. In your experiments, yourepeat edly obtain an (M 1 H)1 value of 767.38. What isthe cause of this discrepancy and what does it tell youabout the region of the protein from which this peptide isderived?arrow_forward
- No plagiarism please. Use your own words. Thanks. Discuss the preferred locations of different classes of amino acids in transmembrane proteins. Explain the formation of thioether-linked prenyl anchor proteins. Explain the structure of caveolae.arrow_forwardBe sure to answer all parts. Give the amino acid sequence of an octapeptide that contains the amino acids Thr, Phe, Ile (2 equiv), Asp, Cys, Val, Leu, and forms the following fragments when partially hydrolyzed with HCl: Thr-Ile-Phe, Phe-Ile-Asp-Val, and Asp-Val-Cys-Leu.arrow_forwardCorrelation between lipid profile and Covid-19.Explain this statement.arrow_forward
- Peptides. 1. Draw the peptide Ala-Glu-Gly-Lys, as it would occur at physiological pH = 7.4. The R groups of Ala and Gly are not acidic or basic, therefore do not have a pka and the charge on these R groups is therefore independent of pH. Glu is acidic and Lys is basic, therefore the charge on these amino acids is pH dependent. The pKas are shown below. pKa N-term = 9.0 C-term = 3.5 Glu4.1 Lys = 10.5 2. Draw a circle around the peptide bonds. 3. Label the C-terminus and the N-terminus. 4. What is the overall charge on the peptide at pH 7.4?arrow_forwardtrue or false1. In the structural characteristics of a nucleotide, the phosphate subunit is bonded to the sugar subunit.2. In the structural characteristics of a nucleotide, the nitrogenous base subunit is bonded to the phosphate subunit.arrow_forwardSubstituent effects. What is the pHPpH of a 0.1 M0.1 M solution of chloroacetic acid (CICH2 COOH, pKa=2.86)? (CICH, COOH, pK, = 2.86) ? doarrow_forward
- cotton. Which aspect of the structure of cellulose accounts for its strength? Cellulose is an abundant structural polysaccharide found in plant cell walls, wood, and interchain hydrogen bonding between extended molecular chains repeating unit of N-acetylglucosamine a(1→4) linkages between glucose units branches that occur every 12-30 residues forms a helical conformation in water Which of the following is not correct about chitin? fundamental constituent of the exoskeletons of crustaceans, insects, and spiders b) chitin chains form extended ribbons that pack side-by side a) similar to cellulose but with repeating units of N-acetyl-D-glucosamine c) d) found mainly in the liver in humans, making up as much as 10% of liver mass stacked sheets of chitin strands are stabilized by intrastrand, interstrand, and intersheet hydrogen bondsarrow_forwardModified TRUE or FALSE. Write the word TRUE if the statement is correct. If the statement is false, write the correct word to make the statement true. 1. The L-form is the naturally-occurring form of amino acids.arrow_forwardBe sure to answer all parts. Give the amino acid sequence of an octapeptide that contains the amino acids Lys, Pro, Tyr, Ile (2 equiv) , Gln, Trp, Phe, and forms the following fragments when partially hydrolyzed with HCl: Lys–Ile-Phe-Pro, Phe-Pro-Gln-Tyr, and Trp-Ile-Lys.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning