Concept explainers
(a)
Interpretation:
The reason by which cell C can grow in the HAT medium is to be determined.
Concept introduction:
HAT medium is the selection medium, used for mammalian cell culture. It is the combination of hypoxanthine, aminopterin, and thymidine. Hypoxanthine and thymidine are the intermediates formed in the DNA synthesis. While aminopterin is the inhibitor of dihydrofolate reductase.
(b)
Interpretation:
Distinction between the normal cells A and the cells that have taken up the foreign DNA is to be determined.
Concept introduction:
HAT medium is the selection medium, used for mammalian cell culture. It is the combination of hypoxanthine, aminopterin, and thymidine. Hypoxanthine and thymidine are the intermediates formed in the DNA synthesis. While aminopterin is the inhibitor of dihydrofolate reductase.
Want to see the full answer?
Check out a sample textbook solutionChapter 25 Solutions
BIOCHEMISTRY-ACHIEVE (1 TERM)
- Enteric bacteria .lactic acid, and propionic bacteria have distinctive metabolic traits that can be used to characterized and identify these organisms .Describe the metabolic characteristics of these organisms name a genus that belong to each group, initiated in what way these organisms can be differentiated.arrow_forwardNeed help, please.arrow_forwardNeed help, please. Drop down answer list choices from left to right. 1. The first set of drop down answer choices are ATP, AMP, cAMP, GTP, GDP, or PP1. 2. The second set of drop down answer choices are activation or inhibition. 3. The third set of drop down answer choices are phosphorylation or dephosphorylation. 4. The fourth set of drop down answer choices are PKA, PP1, GS, or PFK1. 5. The fifth set of drop down answer choices are phosphorylation or dephosphorylation. 6. The sixth set of drop down answer choices are PFK-1, PFK-2, FBase-1, or FBase-2. 7. The seventh set of drop down answer choices are increased activity, decreased activity, or no change in activity.arrow_forward
- ASAP. Explain well. Thank youarrow_forwardBiotransformation. Explain the process of enzyme induction. What are the benefits or down-falls of this process.arrow_forwardNeed help. A protein X binds Ligand Y. The kon characterizing this binding is 1 x 106 M-1s-1, koff is 2 x 10-3 M-1s-1. In your in vitro X-Y binding experiment, at what concentration of Ligand Y half of the Protein X would be bound to Y. Assume that the binding is according to the Lock-and-key modelarrow_forward
- 1G. Please help me in detail. For Molecular Mechanism of ATP versus GTP selectivity of adenylate kinase, Write an expression for the reaction velocity.arrow_forwardCrohn’s disease. Omega-3 fatty acids have been tested asa means to prevent relapse of Crohn’s disease. Two large,randomized, placebo-controlled studies have shown nosuch benefit from omega-3 fatty acids. Suppose you areasked to design an experiment to further study this claim.Imagine that you have collected data on Crohn’s relapsesin subjects who have used these omega-3 fatty acids and similar subjects who have not used them and that you canmeasure incidences of relapse for these subjects. Statethe null and alternative hypotheses you would use in yourstudy.arrow_forwardExplain well. Asaparrow_forward
- Small explanation please. what is a product in the first stage of the Q- cycle? a. two electrons b. Q- cation c. cyt c (oxidized) d. two more protons in the matrix e. Q- radical anionarrow_forwardRequired partner. Aminotransferases require which of the following cofactors: a. NAD+/NADP+NAD+/NADP+ b. Pyridoxal phosphate c. Thiamine pyrophosphate d. Biopterinarrow_forwardpls. help. understand this question and explain step by step. I really wanna get good at this subject so any help with good explanation is greatly appreciatedarrow_forward
- BiochemistryBiochemistryISBN:9781319114671Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.Publisher:W. H. FreemanLehninger Principles of BiochemistryBiochemistryISBN:9781464126116Author:David L. Nelson, Michael M. CoxPublisher:W. H. FreemanFundamentals of Biochemistry: Life at the Molecul...BiochemistryISBN:9781118918401Author:Donald Voet, Judith G. Voet, Charlotte W. PrattPublisher:WILEY
- BiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage LearningBiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage LearningFundamentals of General, Organic, and Biological ...BiochemistryISBN:9780134015187Author:John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. PetersonPublisher:PEARSON