Concept explainers
To determine: How the vector that is engineered to include a reporter gene flanked by a splice acceptor sequence and a polyadenylation signal is used to reveal patterns of gene expression in mammalian cells.
Introduction: Gene trapping is a method of insertional mutagenesis that is designed specifically for the disruption of a gene function. It includes random integration of a reporter gene, referred to as entrapment vector into the genome. It is used to monitor the activity of a gene.
To determine: The reason why gene trapping method depends on the size of a gene’s introns and how the trapped gene is identified.
Introduction: Gene trapping is a method of insertional mutagenesis that is designed specifically for the disruption of a gene function. It includes random integration of a reporter gene referred to as entrapment vector into the genome. It is used to monitor the activity of a gene.
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