Concept explainers
(a)
To determine: The time duration that is required by E.coli RNA polymerase to synthesize the primary transcript for the E.coli gene that encode for lactose
Introduction:
Lac operon is a cell regulatory mechanism that is used to operate the metabolism of lactose in E. coli. The lactose is catabolized by a set of genes known as lac operon. The set of genes present in the lac operon are regulated by regulator gene.
RNA polymerase is a subunit that will catalyze the transcription process in which RNA
(a)
Explanation of Solution
Explanation:
The elongation of the RNA transcript in E.coli will run at the rate of about 50 to 90
Time needed by primary transcript is:
The time can be rounded to 60-100 sec.
Conclusion:
Thus, the time required for primary transcript is 60-100 sec.
(b)
To determine: The length of the transcriptional bubble formed by RNA polymerase.
Introduction:
RNA polymerase is a subunit that will catalyze the transcription process in which RNA polymer is synthesize from the DNA template. RNA polymerase is majorly found in all living organism that takes parts in the central dogma process.
(b)
Explanation of Solution
Explanation:
The elongation of the RNA transcript in E.coli will run at the rate of about 50 to 90 nucleotide per second.
The distance travelled by the replication bubble in 10 sec is:
Conclusion:
Distance travelled by the replication bubble in 10 sec is 500-900nucleotides.
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Chapter 26 Solutions
Lehninger Principles Of Biochemistry 7e & Study Guide And Solutions Manual For Lehninger Principles Of Biochemistry 7e
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