PRINCIPLES OF INSTRUMENTAL ANALYSIS
PRINCIPLES OF INSTRUMENTAL ANALYSIS
7th Edition
ISBN: 9789353506193
Author: Skoog
Publisher: CENGAGE L
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Chapter 26, Problem 26.18QAP
Interpretation Introduction

(a)

Interpretation:

Average number of plates should be calculated.

Concept introduction:

Number of theoretical plates is given by following equation:

N = 16(tRW)2

N = number of plates

tR = retention time

W = width of peak base

Average = NA+NB+NC+ND4

Interpretation Introduction

(b)

Interpretation:

Standard deviation for the N should be calculated.

Concept introduction:

Standard deviation will give how the values deviate from mean value of N.

Interpretation Introduction

(c)

Interpretation:

Average plate height of column should be calculated.

Concept introduction:

N=LH

N = plate count

L = length of the packing (cm)

H = height of the column

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One method for quantitative determination of the concentration of constituents in a sample analyzed by gas chromatography is the area normalization method. In this method, complete elution of all of the sample constituents is necessary. The area of each peak is then measured and corrected for differences in detector response to the different eluates. This correction is accomplished by dividing the area by an empirically determined correction factor. The concentration of the analyte is found from the ratio of its corrected area to the total corrected area of all peaks. For a chromatogram containing three peaks, the relative areas were found to be 16.4, 45.2, and 30.2 in the order of increasing retention time. Calculate the percentage of each compound if the relative detector responses were 0.60, 0.78, and 0.88, respectively
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