Concept explainers
(a)
To write: An equation for mRNA phosphorolysis reaction.
Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and
(b)
To explain: How PNPase accomplish the reverse of phosphorolysis reaction in vitro and how it differs from the reaction carried out by RNA polymerase.
Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.
(c)
To explain: How the property of PNPase having a binding site for long ribonucleotides is an advantage for its primary activity in vivo.
Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.
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