<LCPO> BIOLOGY
12th Edition
ISBN: 9781266216398
Author: Raven
Publisher: MCG CUSTOM
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Chapter 27, Problem 2S
Summary Introduction
To determine:
The reason how the polysaccharide capsule found in the virulent strain of the bacteria is responsible for the cause of pneumonia in mice and justification that
Introduction:
Frederick Griffith conducted the experiments in mice and used two strains of bacteria for infecting the mice namely the rough and smooth strains. The rough strain of the bacteria “Streptococcus” did not cause infection in the mice whereas the smooth strain of the mice has a polysaccharide capsule layer which causes infection in the mice.
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Imagine that you are a student in Alfred Hershey and Martha Chase’s lab in the late 1940s. You are given five test tubes containing E. coli bacteria infected with T2 bacteriophages that have been labeled with either 32P or 35S. Unfortunately, you forget to mark the tubes and are now uncertain about which were labeled with 32P and which with 35S. You place the contents of each tube in a blender and turn it on for a few seconds to shear off the phage protein coats. You then centrifuge the contents to separate the protein coats and the cells. You check for the presence of radioactivity and obtain the following results. Which tubes contained E. coli infected with 32P-labeled phage? Explain your answer. Tube number Radioactivity present in 1 Cells 2 Protein coats 3 Protein coats 4 Cells 5 Cells
Imagine that you are a student in Alfred Hershey and Martha Chase’s lab in the late 1940s. You are given five test tubes containing E. coli bacteria infected with T2 bacteriophages that have been labeled with either 32P or 35S. Unfortunately, you forget to mark the tubes and are now uncertain about which were labeled with 32P and which with 35S. You place the contents of each tube in a blender and turn it on for a few seconds to shear off the phage protein coats. You then centrifuge the contents to separate the protein coats and the cells. You check for thepresence of radioactivity and obtain the following results. Which tubes contained E. coli infected with 32P-labeled phage? Explain your answer.
You are given two samples of DNA, one from Clostridium perfringens and the other from
Mycobacterium tuberculosis. Unfortunately, the labels on the tubes of DNA were accidentally
removed, and you do not know which DNA sample belongs to which organism. As you were
doing some work with Escherichia coli in the lab, you also have some of its DNA available as
well. The %G + %C values for each of the organisms is known (C. perfringens, 27%; M.
tuberculosis, 67%; E. coli, 50%). Based upon this information, how could you quickly
determine the source of the DNA for your two samples?
Chapter 27 Solutions
<LCPO> BIOLOGY
Ch. 27.1 - Prob. 1LOCh. 27.1 - Prob. 2LOCh. 27.1 - Describe classification systems for prokaryotes.Ch. 27.2 - Prob. 1LOCh. 27.2 - Prob. 2LOCh. 27.2 - Prob. 3LOCh. 27.3 - Prob. 1LOCh. 27.3 - Prob. 2LOCh. 27.3 - Prob. 3LOCh. 27.4 - Describe the metabolic strategies employed by...
Ch. 27.4 - Compare and contrast the ways prokaryotes can...Ch. 27.5 - Describe the role of prokaryotes in biogeochemical...Ch. 27.5 - Prob. 2LOCh. 27.5 - Prob. 3LOCh. 27.6 - Prob. 1LOCh. 27.6 - Prob. 2LOCh. 27.6 - Prob. 3LOCh. 27 - Data analysis If the excision of an F plasmid Is...Ch. 27 - Prob. 1IQCh. 27 - Prob. 1UCh. 27 - A bacteria that obtains carbon from the atmosphere...Ch. 27 - Prob. 3UCh. 27 - Bacteria and archaebacteria may be distinguished...Ch. 27 - Prob. 5UCh. 27 - Prob. 6UCh. 27 - Prob. 7UCh. 27 - Prob. 1ACh. 27 - Prob. 2ACh. 27 - Prob. 3ACh. 27 - Prob. 4ACh. 27 - Prob. 5ACh. 27 - Some bacteria are capable of forming NH4+ from...Ch. 27 - Prob. 7ACh. 27 - Prob. 1SCh. 27 - Prob. 2SCh. 27 - Use of multiple antibiotics is not a bad idea if...Ch. 27 - Soil-based nitrogen-fixing bacteria appear to be...
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