FUND.OF GENERAL,ORG.+BIO.CHEM.(LL)-PKG
8th Edition
ISBN: 9780134773216
Author: McMurry
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 27.3, Problem 27.3CIAP
Interpretation Introduction
Interpretation:
Why Taq polymerase used in PCR instead of the DNA polymerase found in humans has to be explained.
Concept Introduction:
Polymerase chain reaction (PCR):
Polymerase chain reaction (PCR) is a technique which is used for amplification of DNA strand by thermos aquaticus polymerase (Taq polymerase).
Taq polymerase is an enzyme which is used in the PCR reaction because it can withstand very high temperatures because PCR reaction involves different temperatures; denaturation temperature (
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
Why is it necessary to use a special DNA polymerase(Taq polymerase) in PCR?
Why is Taq polymerase especially useful for PCR?
Most PCR reactions do not use the more expensive types of DNA polymerase, which have DNA proofreading. How might this be a problem in accurately copying specific DNA sequences of the target gene?
Chapter 27 Solutions
FUND.OF GENERAL,ORG.+BIO.CHEM.(LL)-PKG
Ch. 27.1 - Decode the following sequence of letters to find...Ch. 27.3 - Prob. 27.1CIAPCh. 27.3 - Prob. 27.2CIAPCh. 27.3 - Prob. 27.3CIAPCh. 27.3 - Prob. 27.2KCPCh. 27.4 - Prob. 27.3PCh. 27.4 - A restriction enzyme known as EcoRI cuts DNA in...Ch. 27.4 - Prob. 27.5PCh. 27.5 - Classify the following activities according to the...Ch. 27.5 - Prob. 27.4CIAP
Ch. 27.5 - Prob. 27.5CIAPCh. 27.5 - Prob. 27.6CIAPCh. 27 - What steps are necessary in the mapping of the...Ch. 27 - Prob. 27.8UKCCh. 27 - List the four types of noncoding DNA (see Section...Ch. 27 - In general, what are the differences between...Ch. 27 - What is recombinant DNA? How can it be used to...Ch. 27 - Identify some major potential benefits of the...Ch. 27 - Prob. 27.13APCh. 27 - Prob. 27.14APCh. 27 - Prob. 27.15APCh. 27 - Prob. 27.16APCh. 27 - Prob. 27.17APCh. 27 - Prob. 27.18APCh. 27 - Prob. 27.19APCh. 27 - You may have heard of Dolly, the cloned sheep...Ch. 27 - Prob. 27.21APCh. 27 - Prob. 27.22APCh. 27 - What is the role of the enzyme telomerase? In what...Ch. 27 - Prob. 27.24APCh. 27 - Prob. 27.25APCh. 27 - Prob. 27.26APCh. 27 - Prob. 27.27APCh. 27 - What is a SNP?Ch. 27 - How are SNPs linked to traits in individual human...Ch. 27 - List some potential biological effects of SNPs.Ch. 27 - Prob. 27.31APCh. 27 - Prob. 27.32APCh. 27 - Prob. 27.33APCh. 27 - Prob. 27.34APCh. 27 - Prob. 27.35APCh. 27 - Prob. 27.36APCh. 27 - Prob. 27.37APCh. 27 - Prob. 27.38APCh. 27 - In the formation of recombinant DNA. a restriction...Ch. 27 - Give the sequence of unpaired bases that would be...Ch. 27 - Are the following base sequences sticky or not...Ch. 27 - Prob. 27.42APCh. 27 - Prob. 27.43APCh. 27 - Provide two examples of genetically engineered...Ch. 27 - Prob. 27.45APCh. 27 - Why is the field of bioethics so important in...Ch. 27 - Prob. 27.47CPCh. 27 - Prob. 27.48CPCh. 27 - Prob. 27.49CPCh. 27 - Prob. 27.50CPCh. 27 - What is a restriction endonuclease?Ch. 27 - Prob. 27.52CPCh. 27 - Prob. 27.53GPCh. 27 - One of the most actively pursued areas in genomics...Ch. 27 - Prob. 27.55GP
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.Similar questions
- What is the difference between reverse transcriptase PCR(RT-PCR) and standard PCR? For what purpose would youuse RT-PCR?arrow_forwardDuring PCR amplification in preparation for DNA sequencing, why were there different colors at the 3’ ends of the fragments produced? (What did these four colors represent?)arrow_forwardAfter four cycles of PCR, which type of PCR product predominates? Explain why.arrow_forward
- Can you explain how PCR( Polymerase Chain Reaction can detect very) low amounts of DNA?arrow_forwardWhy Touch-down PCR technique is better than the Gradient PCR technique? Also, from those techniques which technique produces the pure product?arrow_forwardWhat properties of DNA does PCR take advantage of?arrow_forward
- Why do you think the DNA is stored cold with the InstaGene matrix after boiling the samples and during subsequent steps in DNA Abstraction of PCR Bioinformatics?arrow_forwardWhat is the purpose of including a PCR reaction with no added DNA? Isn’t this just a waste of expensive enzyme when we know that without template, you will not get any PCR products?arrow_forwardHow is DNA amplified in a polymerase chain reaction(PCR) procedure?arrow_forward
- . In the PCR process, if we assume that each cycle takes5 minutes, how manyfold amplification would be accomplished in 1 hour?arrow_forwardAfter four cycles of PCR, which products predominate? Explain why.arrow_forwardIn rRT–PCR, why do need to convert RNA to DNA first? Why can’t you amplify the RNA molecule immediately and detect the presence of the gene?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Biology Today and Tomorrow without Physiology (Mi...BiologyISBN:9781305117396Author:Cecie Starr, Christine Evers, Lisa StarrPublisher:Cengage LearningHuman Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage Learning
Biology Today and Tomorrow without Physiology (Mi...
Biology
ISBN:9781305117396
Author:Cecie Starr, Christine Evers, Lisa Starr
Publisher:Cengage Learning
Human Heredity: Principles and Issues (MindTap Co...
Biology
ISBN:9781305251052
Author:Michael Cummings
Publisher:Cengage Learning
QCE Biology: Introduction to Gene Expression; Author: Atomi;https://www.youtube.com/watch?v=a7hydUtCIJk;License: Standard YouTube License, CC-BY