Chapter 28, Problem 11P

Summary Introduction

To draw: The diagram of different functional domains that would be found in the Gal4 protein and in the engineered protein.

Introduction:

The gal operon is present in yeast and regulates the galactose metabolism, by encoding the enzymes which are necessary for the process. Gal operon contain O_E and O_I operator site, present before the promoter.

Summary Introduction

To determine: The reason that engineered protein no longer regulates transcription of GAL genes.

Introduction:

DNA sequence contain DNA binding domain for binding of proteins involve in gene regulation. RNA polymerase binds to DNA binding protein, and facilitates the activator proteins to initiate the transcription.

Summary Introduction

To determine: The mechanism by which DNA binding site by this chimeric protein to make it functional in activating transcription of GAL genes

Introduction:

DNA sequence contain DNA binding domain for binding of proteins involve in gene regulation. RNA polymerase binds to DNA binding domain, and facilitates the activator proteins to initiate the transcription.