Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Textbook Question
Chapter 2.8, Problem 2MQ
Chapter Review
- Why would it be impossible for gram-positive bacteria to store sulfur as gram-negative sulfur-oxidizing chemolithotrophs can?
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Q8) What type of molecule is found in the cell wall of acid-fast bacteria that makes Gram staining impossible?
-What is the medical importance of the capsule? (why would pathogens with a capsule be harder to kill/neutralize?)
Q2) How could you determine if the cloudiness (turbidity) of your nutrient broth was from a mixture of microbes or from the growth of only one kind of microbe?
- What is one of the main benefits of an agar?
- What is the purpose of flaming the loop before use? After use?
- How do you know if your bacteria can move from the ‘agar deep’?
- Why is aseptic transfer technique important?
Topic: Multiple Tube Fermentation
Questions:
1. What protocol should you follow if gas formation is less than 10%?
a. Why not test for pathogens directly instead of usimg indicator organism such as coliform bacteria?
b. Can you use the MPN method to test for the presence of coliforms in food? Why or why not?
c. Describe another method of determining the presence of coliforms in a water sample.
Chapter 2 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 2.1 - How do cocci and rods differ in morphology?Ch. 2.1 - Using a microscope, could you differentiate a...Ch. 2.1 - What are the major morphologies of prokaryotic...Ch. 2.2 - What physical property of cells increases as cells...Ch. 2.2 - How can the small size and haploid genome of...Ch. 2.2 - What are the approximate limits to how small a...Ch. 2.2 - How large can a bacterium be? How small? Why is it...Ch. 2.3 - Draw the basic structure of a lipid bilayer and...Ch. 2.3 - Prob. 2MQCh. 2.3 - Prob. 3MQ
Ch. 2.3 - Describe in a single sentence the structure of a...Ch. 2.4 - Why do bacterial cells need cell walls? Do all...Ch. 2.4 - Prob. 2MQCh. 2.4 - What do the enzyme lysozyme and the antibiotic...Ch. 2.4 - Prob. 1CRCh. 2.5 - Prob. 1MQCh. 2.5 - Prob. 2MQCh. 2.5 - Prob. 3MQCh. 2.5 - List several functions of the outer membrane in...Ch. 2.6 - Prob. 1MQCh. 2.6 - Prob. 2MQCh. 2.6 - Prob. 1CRCh. 2.7 - Prob. 1MQCh. 2.7 - Prob. 2MQCh. 2.7 - Chapter Review How can type IV pili facilitate...Ch. 2.7 - Prob. 1CRCh. 2.8 - Prob. 1MQCh. 2.8 - Chapter Review Why would it be impossible for...Ch. 2.8 - Chapter Review How are magnetosomes and the...Ch. 2.8 - Prob. 1CRCh. 2.9 - Prob. 1MQCh. 2.9 - Prob. 2MQCh. 2.9 - Prob. 1CRCh. 2.10 - Prob. 1MQCh. 2.10 - Prob. 2MQCh. 2.10 - Prob. 3MQCh. 2.10 - In a few sentences, indicate how the bacterial...Ch. 2.11 - Prob. 1MQCh. 2.11 - Prob. 2MQCh. 2.11 - Prob. 1CRCh. 2.12 - Prob. 1MQCh. 2.12 - Prob. 2MQCh. 2.12 - Contrast the mechanism for motility in...Ch. 2.13 - Prob. 1MQCh. 2.13 - Prob. 2MQCh. 2.13 - Prob. 3MQCh. 2.13 - Chapter Review How does scotophobotaxis differ...Ch. 2.13 - In a few sentences, explain how a swimming...Ch. 2.14 - Prob. 1MQCh. 2.14 - Prob. 2MQCh. 2.14 - Prob. 3MQCh. 2.14 - List at least three features of eukaryotic cells...Ch. 2.15 - Prob. 1MQCh. 2.15 - Prob. 2MQCh. 2.15 - Prob. 3MQCh. 2.15 - How are the mitochondrion and the hydrogenosome...Ch. 2.16 - Prob. 1MQCh. 2.16 - Prob. 2MQCh. 2.16 - Prob. 3MQCh. 2.16 - Describe the major functions of the endoplasmic...Ch. 2 - Prob. 1AQCh. 2 - Assume you are given two cultures, one of a...Ch. 2 - Prob. 3AQ
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- Q2) How could you determine if the cloudiness (turbidity) of your nutrient broth was from a mixture of microbes or from the growth of only one kind of microbe? - How do you know if your bacteria can move from the ‘agar deep’? - Why is aseptic transfer technique important?arrow_forwardquestion- Can lactobacillus bacteria (found in human digestive and urinary tracts) be considered an extremophiles?arrow_forwardQ -9 Outline how the electron microscope has increased our knowledge of cell structure.arrow_forward
- Q13) Explain the similarities and differences between column chromotography and gel electrophoresis?arrow_forwardhttps://courses.lumenlearning.com/microbiology/chapter/controlling-microbial-growth/ Read this Article about Controlling Microbial Growth and answer the following questions: Which is most effective at removing microbes from a product: sanitization, degerming, or sterilization? Explain. What are two possible reasons for choosing a bacteriostatic treatment over a bactericidal one? Name at least two factors that can compromise the effectiveness of a disinfecting agent.arrow_forwardxplain the four stages of the growth curve for a microorganism. What is the generation time? Which aspect of the growth curve is used to calculate the generation time and why? How is the generation time calculated and why does it make sense that E.coli and M.tuberculosis have such drastically different generation times?arrow_forward
- Q7) Evolutionarily, why are antibiotics produced? How concentrated are microbes in the soil (citation for this one please)? Knowing that, why do you think the soil is the most likely place to find antibiotics, compared to other environments?arrow_forwardQ1 How can you measure the activity of actinomycetes that produce antibiotics ?Q2 What is the reason of the production of clear zone around the proteolytic bacteria when grown on casein agar ? Q3 Why some bacteria produce Nanoparticles when grown on trace elements? Q4 Why the ureolytic bacteria use urea? And why they produce CaCO3?arrow_forwardQ4. Why are some plates placed into a candle jar? Q6. What is the purpose of each kind of medium?arrow_forward
- Topic 1: Gram staining principle a. Describe the structure of peptidoglycan b. Describe in detail the architecture and structure of Gram negative cell wall structure c. Discuss how Gram positive cell wall is different from Gram negative wall structure. d. What are teichoic acid and their location? Provide the role of the teichoic acid in bacterial cells.arrow_forwardQ5) Since KOH can also determine whether a culture is Gram positive or negative, why go through all the trouble of doing a Gram stain instead? - What causes the KOH test to work? - How do you know a KOH test has identified a gram negative? Gram positive?arrow_forwardQ3) For EMB (Eosin Methylene Blue) agar, what result ‘confirms’ coliforms (positive test)? - The MPN mostly looks to grow E. coli as the indicator species of fecal contamination. Most E. coli is not pathogenic, and you can get many more harmful things like viruses from fecal contamination. Why is E. coli the only thing we look for in the lab to determine whether water is contaminated with fecal matter? (like why not look for the viruses?)arrow_forward
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