Microbiology: An Evolving Science (Fourth Edition)
Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 28, Problem 9RQ
Summary Introduction

To review:

The procedure of RT-qPCR (reverse transcription-quantitative polymerase chain reaction).

Introduction:

Polymerase chain reaction is a method that is used in molecular biology for the amplification of DNA (deoxyribonucleic acid) molecules. It requires a DNA template, DNA polymerase enzyme, and the Taq (derived from Thermus aquaticus) polymerase enzyme. RT-qPCR converts the RNA (ribonucleic acid) into a cDNA (complementary DNA) template with the use of reverse transcriptase enzyme.

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Molecular Techniques: Basic Concepts; Author: Dr. A's Clinical Lab Videos;https://www.youtube.com/watch?v=7HFHZy8h6z0;License: Standard Youtube License