COWAN CONNECT ACCESS CARD MICROBIOLOGY:
4th Edition
ISBN: 9781265200015
Author: Cowan
Publisher: MCG
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Textbook Question
Chapter 3, Problem 15TF
The best stain to use to visualize a microorganism with a large capsule is a simple stain.
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Additional Differential Staining Procedures: Negative stain, Capsule Stain, Endospore stain, Flagella stain. What is a positive and negative results of this procedures?
Draw what you would expect to see for acid fast stain and endospore stain procedure if you did not stain with counterstain. Color pencils need to be used.
Which of the following serves as a counterstain for the gram-stain technique? It also happens to be the counterstain for the endospore staining technique.
Chapter 3 Solutions
COWAN CONNECT ACCESS CARD MICROBIOLOGY:
Ch. 3.1 - Prob. 2CFCh. 3.1 - Explain what the Five Is mean and what each step...Ch. 3.1 - Discuss three physical states of media and when...Ch. 3.1 - Compare and contrast selective and differential...Ch. 3.1 - Provide brief definitions for defined and complex...Ch. 3.2 - Prob. 5AYPCh. 3.2 - Prob. 6AYPCh. 3.2 - Prob. 7AYPCh. 3.2 - Prob. 8AYPCh. 3.2 - Compare and contrast the three main categories of...
Ch. 3 - Prob. 1CFCh. 3 - Prob. 1MCQCh. 3 - A mixed culture is a. the same as a contaminated...Ch. 3 - Resolution is ____ with a longer wavelength of...Ch. 3 - A real image is produced by the a. ocular. b....Ch. 3 - Prob. 5MCQCh. 3 - The specimen for an electron microscope is always...Ch. 3 - Prob. 7MCQCh. 3 - Bacteria tend to stain more readily with cationic...Ch. 3 - Multiple Matching. For each type of medium, select...Ch. 3 - A fastidious organism must be grown on what type...Ch. 3 - Agar has the disadvantage of being easily...Ch. 3 - A subculture is a culture made from an isolated...Ch. 3 - Prob. 13TFCh. 3 - Prob. 14TFCh. 3 - The best stain to use to visualize a microorganism...Ch. 3 - Prob. 1CTQCh. 3 - Prob. 2CTQCh. 3 - Prob. 3CTQCh. 3 - What is the functional type of mannitol salt agar...Ch. 3 - Create a short paragraph to differentiate among...Ch. 3 - Prob. 6CTQCh. 3 - Prob. 7CTQCh. 3 - a.Create a paragraph to differentiate among the...Ch. 3 - Prob. 9CTQCh. 3 - You are a scientist studying a marsh area...Ch. 3 - Prob. 1CCCh. 3 - Prob. 2CCCh. 3 - Prob. 3CCCh. 3 - Prob. 4CCCh. 3 - Prob. 5CCCh. 3 - Visual Connections Blooms Level 5: Evaluate These...Ch. 3 - Prob. 1CM
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- Explain why it is important to use only a small amount of bacteria when preparing a smear. What are the two things that are stained in a capsule stain? What is NOT stained in a capsule stain?arrow_forwardWhat stain technique is used here? What is the primary stain, mordant, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with purple color. Describe the organism with pinkish red color.arrow_forwardWhy is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?arrow_forward
- Name a few group of microorganisms where Grams staining will not work and/or not appropriate to use. Why?arrow_forwardIs is best to observe slides of bacteria stained with the acid fast stain under high dry magnification. True or falsearrow_forwardDescribe the bacteria observed in different staining procedures. Please refer to the photo - Direct Stain - Negative Stainarrow_forward
- Choose the dyes or reagents used in the Endospore stain. (More than one choices are correct) a acid alcohol b crystal violet c carbolfuschin d malachite green e safranin f iodine g alcohol h methylene bluearrow_forwardWhich of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.arrow_forwardHow does smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium? Mark all that apply: A smear made from liquid media will be thinner than a smear made from solid media A smear made from solid media doesn't have to be fixed but a smear from liquid media does A smear made from liquid media will be more thick than a smear made from solid Water must be added to a smear made from solid media but not liquid media A smear made from liquid media doesn't have to be fixed but a smear from solid media does Water must be added to a smear from liquid media but not from solidarrow_forward
- Explain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a dayarrow_forwardWhat stain technique must this be? What is used to stain the background? What is used to stain the bacterial cell (in our method)? What structure is the clear (white) layer? Do we heat fix this? Capsule stain, No heat fixation, crystal violet, copper sulfate,arrow_forwardCreate paragraphs to differentiate among the following terms: positive stain, negative stain, simple stain, and differential stain. please provide minimum of 400 wordsarrow_forward
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