Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Videos
Textbook Question
Chapter 3, Problem 1CA
The effectiveness of sanitation procedures in hospitals is determined by sampling surfaces for bacteria. Samples can be taken by rubbing a sterile, moistened cotton swab on a surface and inoculating a nutrient agar plate or with a Rodac environmental sampling plate. The 67-mm Rodac plates are designed so that an agar medium can be overfilled, producing a dome-shaped surface that can be pressed onto a surface for sampling microbes. As the sanitation control officer, you test each method to determine which you will use. What can you conclude from these results?
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows.
Nutrient broth: 8 g/liter
Nutrient agar: 28 g/liter
Answer the following:
a. What is the weight in grams of nutrient broth?
b. What is the weight in grams of nutrient agar?
c. What is the distilled water in mL for nutrient broth?
d. What is the distilled water in mL for nutrient agar?
Which one of the following microorganisms would you use as a quality control check to ensure that your sterile technique method is properly working?
S. aureus, a gram-positive bacterium
E. coli, a gram-negative bacterium
B. cereus, an endospore-forming bacterium
M. leprae, an acid-fast bacterium
T. pallidum, a flagellated, disease causing bacterium
A patient is diagnosed with a urinary tract infection and a urine sample is obtained and sent to the microbiology lab. The urine sample is inoculated to agar plates and after 24 hours of incubation bacterial colonies are present on the plates.
Draw what the bacterial growth on the agar plate looks like.
Chapter 3 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. What must be done with the bacterial sample after it is processed in the sterilizer?arrow_forwardA surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. Which type of bacteria would be used to test the autoclave?arrow_forwardA microbe is growing on Sabouraud dextrose agar, which has a slightly acidic pH. Which of the following is this microbe likely to be? a Gram-negative bacterium an acid-fast bacterium a fungus a Gram-positive bacteriumarrow_forward
- A broth culture is initially inoculated with a single bacterial cell. For the first hour the bacteria has a generation time of 30 minutes, for the second hour the generation time has been reduced to 15 minutes, and for the third hour the generation time has been reduced to 10 minutes. At the end of the third hour how many bacteria are present in the broth culture?arrow_forwardWhich isolation technique is best used in isolating microorganisms (in countable good plates) from a gram of soil sample with expectedly high microbial count? A. Streak Plate technique B. Serial Dilution-Pour Plate technique C. Spread Plate technique D. Hanging Drop technique 2. Which materials are needed in isolating bacteria using Streak Plate technique? Check all that apply. ( )nutrient agar plate ( ) inoculating loop ( ) microbial inoculum ( ) flame source 3. Which term refers to that solution of sterile distilled water/normal saline/peptone water used to "thin out" dense microbial populations in Serial Dilution-Pour Plate technique used in the isolation of microorganisms? A. inoculum B. culture medium C. diluent D. microbial culturearrow_forwardNoah wanted to transfer Staphylococcus aureus from a broth to an agar plate. He picked up the broth culture, removed the cap, and flamed the mouth of the tube. He inserted an inoculating loop to obtain a bacterial sample. Then, he flamed the mouth of the tube and replaced the cap. Noah opened the lid of a labeled agar plate diagonally and used the loop to streak the surface of the agar. After closing the lid, he flamed the loop in an incinerator and put it back in its container. The plate was incubated upside down for 24–48 hours. What did Noah do wrong in this transfer? a. He did not use the transfer tool correctly. b. He did not handle the culture tube correctly. c. He did not handle the agar plate correctly. d. He used the wrong tool in transfer.arrow_forward
- Assuming you are working in a microbiology laboratory and a client sent you a sample for the following analyses: Identification of all microorganisms in the soil sample Enumeration of culturable bacteria in the sample Enumeration of both culturable and non-culturable bacteria in the sample For a to c, explain the following: Method of analyses to be used Step by step report on how the analyses will be conductedarrow_forwardAs a microbiology student, how can you prevent or control coliform contamination in drinking water of your respective communityarrow_forwardduring inoculation, the bacterial culture tube is always held at an angle and the lid of the Petri dish is slightly open. Explain the purpose of these steps briefly.arrow_forward
- A microbiologist used the concept of enrichment culture to isolate aerobic and anaerobic nitrogen-fixing bacteria, sulfate-reducing bacteria, and sulfur-oxidizing bacteria. What kind of selective media could he haveused for isolating each of these four classes of microbes?arrow_forward“Salt agar” is a type of ______ medium for culturing some microbes and not othersarrow_forwardGiven the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams). Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Formula: C1V1 = C2V2 *Concentration *Volume Computation: What are the answers to the following. Weight in grams of nutrient broth: _________ Distilled water in mL for nutrient broth: __________ Weight in grams of nutrient agar __________ Distilled water in mL for nutrient agar: ____________arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Comprehensive Medical Assisting: Administrative a...NursingISBN:9781305964792Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy CorreaPublisher:Cengage Learning
Basic Clinical Lab Competencies for Respiratory C...NursingISBN:9781285244662Author:WhitePublisher:Cengage
Microbiology for Surgical Technologists (MindTap ...BiologyISBN:9781111306663Author:Margaret Rodriguez, Paul PricePublisher:Cengage Learning
Comprehensive Medical Assisting: Administrative a...
Nursing
ISBN:9781305964792
Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa
Publisher:Cengage Learning
Basic Clinical Lab Competencies for Respiratory C...
Nursing
ISBN:9781285244662
Author:White
Publisher:Cengage
Microbiology for Surgical Technologists (MindTap ...
Biology
ISBN:9781111306663
Author:Margaret Rodriguez, Paul Price
Publisher:Cengage Learning
Infection Prevention and Control; Author: thecityoftoronto;https://www.youtube.com/watch?v=jx9sRYmBW3Q;License: Standard Youtube License