To calculate: The percentage of amino acids liberated in the fourth cycle would be leucine if each Edman cycle is 96% efficient.
Introduction:
Edman degradation is a method of protein sequencing developed by Pehr Edman. Edman reagent (phenylisothiocyanate) is used in this process. The amino terminal of the peptide sequence is labeled and Edman reagent is used to cleave the N-terminal from the peptide.
To calculate: The percentage of amino acids liberated in the fourth cycle would be leucine if each Edman cycle is 99% efficient.
Introduction:
Edman degradation is a method of protein sequencing developed by Pehr Edman. Edman reagent (phenylisothiocyanate) is used in this process. The amino terminal of the peptide sequence is labeled and Edman reagent is used to cleave the N-terminal from the peptide.
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