Concept explainers
(a)
To describe: The products of a chain-terminator sequencing technique in which too few are present.
Concept introduction: The chain termination method or Sanger sequencing method was devised by Frederick Sanger in 1977. In this technique, the template DNA to be sequenced is incubated in a tube with DNA-polymerase, an appropriate primer, and DNA
(b)
To describe: The products of a chain-terminator sequencing procedure in which an excess of primers is present.
Concept introduction: The chain termination method or Sanger sequencing method was devised by Frederick Sanger in 1977. In this technique, the template DNA to be sequenced is incubated in a tube with DNA-polymerase, a suitable primer, and the four DNA nucleotides (dATP, dTTP, dGTP, and dCTP) for the polymerization reaction.
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