Concept explainers
(a)
To describe: The possible products of a PCR experiment which carry a single stranded disruption in the target DNA sequence, which is present in only one copy in the starting sample.
Concept introduction:
The
(b)
To describe: The possible products of a PCR experiment which carry a double stranded break in the target DNA sequence, which is present in only one copy in the starting sample.
Concept introduction:
The polymeric chain reaction (PCR) is a technique in which amplification of a specific DNA segment occurs quickly and accurately. In this technique, a DNA sample is separated by using restriction enzymes into single strands. Then these strands are incubated with DNA polymerase such as Taq polymerase, two primers, and deoxynucleotides triphosphate (dNTPs). A PCR cycle consists of three steps such as strand separation, the hybridization of primers, and the extension of by DNA synthesis.
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Chapter 3 Solutions
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