Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Question
Chapter 31, Problem 17P
Interpretation Introduction
Interpretation:
The use of FRET in conformational changes in protein bound to chaperonins like GroEL needs to be explained.
Concept Introduction :
FRET stands for Fluorescence Resonance Energy Transfer, which is a physical phenomenon initially defined 50 years ago and today used in research and drug detection very frequently. Fluorescence Resonance Energy Transfer depends on the distance between the donor and acceptor molecule between which the energy is transferred.
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resting state of the protein, the Lys 216 Schiff base has pKa = 9.5 and Asp 85 has pKa = 3.5. When the conformational change occurs, the proton that was on the Schiff base moves to Asp 85. This should tell you that one or both of these two pKas
changed with the conformational change. How does the pKa of Asp 85 compare with the pKa of the Lys 216 Schiff base after the conformational change?
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