BIOLOGY
BIOLOGY
12th Edition
ISBN: 9781260169614
Author: Raven
Publisher: RENT MCG
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Chapter 5, Problem 1S

figure 5.5 describes a classic experiment demonstrating the ability of proteins to move within the plane of the cell’s plasma membrane. The following table outlines three different experiments using the fusion of labeled mouse and human cells.

Experiment Conditions Temperature (°C) Result
1 Fuse human and mouse cells 37 Intermixed membrane proteins
2 Fuse human and mouse cells in presence of ATP inhibitors 37 Intermixed membrane proteins
3 Fuse human and mouse cells 4 No intermixing of membrane proteins

What conclusions can you reach about the movement of these proteins?

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Which of the following best describes an integral membrane protein? A.A protein that has an extracellular domain, a membrane-spanning domain that spans the lipid bilayer of the plasma membrane, and an intracellular domain. B.A protein that peripherally associates with the membrane via ionic interactions another protein embedded in the membrane. C.A protein that is soluble in the cytoplasm. D.A protein that peripherally associates with the membrane via ionic interactions between polar charged amino acids and the polar head groups of the phospholipids in the membrane.
Which of the following would be most likely to interfere with the proper insertion of a protein into the plasma membrane? Select the best answer.    Options:   The switch of a polar amino acid to a non-polar in a transmembrane region of the protein.   The switch of a non-polar amino acid to a polar in a transmembrane region of the protein.   The switch of a non-polar amino acid to a polar in a cytoplasmic region of the protein.   The switch of a polar amino acid to a non-polar in a cytoplasmic region of the protein.
When rhodamine-dyed mouse proteins were first mixed with fluorescein-dyed human proteins (in mouse/human hybrid cell fusions), these proteins appeared to exhibit:  restricted movement, based on confinement by diffusion barriers restricted movement, based on tethering to extracellular molecules unrestricted movement, similar to membrane phospholipids restricted movement, based on anchoring to intracellular proteins restricted movement, based on attachment to other cells
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The Cell Membrane; Author: The Organic Chemistry Tutor;https://www.youtube.com/watch?v=AsffT7XIXbA;License: Standard youtube license