(a)
To tabulate: The values of specific activity, %yield, and fold purification for the crude extract, DEAE-cellulose chromatography, and affinity chromatography from the given values of protein concentration.
Concept introduction: DEAE (Diethylaminoethyl) cellulose chromatography is an ion exchange chromatography used for the separation and purification of proteins. DEAE cellulose is a weak anion exchanger, so the negatively charged protein binds to DEAE. It separates the substance based on its charges such that a negatively charged protein binds with the positively charged DEAE. Affinity chromatography is a separation method based on interactions. This is the efficient purification method for complex proteins and other complexes.
(b)
To determine: The step in which DEAE or affinity chromatography causes the greatest loss of myoglobin in the given table.
Concept introduction: DEAE (Diethylaminoethyl) cellulose chromatography is an ion exchange chromatography used for the separation and purification of proteins. DEAE cellulose is a weak anion exchanger, so the negatively charged protein binds to DEAE. It separates the substance based on their charges, where a negatively charged protein binds with the positively charged DEAE. Affinity chromatography is a separation method based on the interactions. This is the efficient purification method for complex proteins and other complexes.
(c)
To explain: The step in which DEAE or affinity chromatography causes greater purification is given in the table.
Concept introduction: DEAE (Diethylaminoethyl) cellulose chromatography is an ion exchange chromatography used for the separation and purification of proteins. DEAE cellulose is a weak anion exchanger, so the negatively charged protein binds to DEAE. It separates the substance based on its charges where a negatively charged protein binds with the positively charged DEAE. Affinity chromatography is a separation method based on interactions. This is the efficient purification method for complex proteins and other complexes.
(d)
To explain: The best step that could use for the purification of myoglobin.
Concept introduction: DEAE (Diethylaminoethyl) cellulose chromatography is an ion exchange chromatography used for the separation and purification of proteins. DEAE cellulose is a weak anion exchanger, so the negatively charged protein binds to DEAE. It separates the substance based on its charges, where a negatively charged protein binds with the positively charged DEAE. Affinity chromatography is a separation method based on interactions. This is the efficient purification method for complex proteins and other complexes.
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