LIFE:SCIENCE OF BIOL.(LL) >CUSTOM<
11th Edition
ISBN: 9781319209957
Author: Sadava
Publisher: MAC HIGHER
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Chapter 5, Problem 3Q
Summary Introduction
To review:
Whether something different is learned by researchers by using the method of cell fractionation, which is used to determine, the path of tagged protein.
Introduction:
Cell fractionation is the method, by which cells are fractured or opened so that protein expression of the cellular components can be determined. The cells, which are infected by a fluorescently labeled virus, are first subjected to homogenization so that the cellular components like mitochondria, nucleus, and endoplasmic reticulum come inside the homogenization mixture.
The homogenized mixture is then checked for the expression of proteins at different times. The green fluorescent protein (GFP) is also used to check for the presence of different proteins in the cellular components.
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