BIOCHEMISTRY-ACHIEVE (1 TERM)
9th Edition
ISBN: 9781319402853
Author: BERG
Publisher: MAC HIGHER
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Question
Chapter 5, Problem 7P
Interpretation Introduction
Interpretation:
The reason for the given statement should be explained.
Concept introduction:
DNA stands for deoxyribonucleic acid, is a biological macromolecule. DNA contains double helical strands along with the complementary base pairs. The four complementary bases of DNA are adenine (A), thymine (T), guanine (G) and cytosine (C). In the formation of recombinant DNA, the restriction enzymes are involved to cut the particular region in the DNA molecule. This region is known as restriction site.
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Restriction sites of Lambda (A) DNA - In base pairs (bp)
The sites at which each of the 3 different enzymes will cut the same strand of lambda DNA
are shown in the maps (see figure 3 B-D), each vertical line on the map is where the respective
enzymes will cut.
A DNA
A
(bp)
48502
10 000
20 000
30 000
40 000
9162
17 198
B
Sal I
7059
14 885
28 338
35 603
42 900
(bp)
Hae III
11 826
21 935
29 341
38 016
(bp)
11648
29,624
Eco R1
(bp)
10 592 16 246
28 915
41 864
Figure 3: Restrictrion site map showing the following A) inear DNA that is not cut as reference B) DNA CLt with Sal L C) DNA cut with Hae , D)
DNA cut with Eco RI
1. Calculate the size of the resulting fragments as they will occur after digestion and write
the sizes on the maps below. Note that linear DNA has a total size of 48 502 bp (see
figure 3A).
Page 3 of 7
9162
17 198
Sal i
(bp)
7059
14 885
28 338
35 603
42 900
Hae I
(bp)
11 826
21 935
29 341
38 016
11648
29,624
Eco R1
(bp)
10 592
16 246
28 915
41 864
please help me with this question.
As this is a non-directional cloning, recombinant plasmids can contain an insert ligated into the vector in two different orientations. Provide two diagrams to illustrate the two potential recombinant plasmids, with the inserts ligated in opposite orientations. Include all RE sites and distances between sites on the diagram.
Backward? Bacteriophage T7 helicase moves along DNA in the 5'-to-
3'5'-to-3' direction. Other helicases have been reported to move in
the 3'-to-5'3'-to-5' direction. Is there any fundamental reason why
you would expect helicases to move in one direction or the other?
Chapter 5 Solutions
BIOCHEMISTRY-ACHIEVE (1 TERM)
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