EBK CAMPBELL BIOLOGY
10th Edition
ISBN: 9780136539414
Author: Reece
Publisher: VST
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Chapter 56.4, Problem 3CC
MAKE CONNECTIONS → Mutagens are chemical and physical agents that induce mutations in DNA (see Concept 17.5). How does reduced ozone concentration in the atmosphere increase the likelihood of mutations in various organisms?
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E17. Gene mutagenesis is also used to explore the structure and function
of proteins. For example, changes can be made to the coding
sequence of a gene to determine how alterations in the amino acid
sequence affect the function of a protein. Let's suppose that you are
interested in the functional importance of a particular glutamic acid
(an amino acid) within a protein you are studying. By site-directed
mutagenesis, you make mutant proteins in which this glutamic
acid codon has been changed to other codons. You then test the
encoded mutant proteins for functionality. The results are as follows:
Functionality (%)
Normal protein
100
Mutant proteins containing
Тугosine
Phenylalanine
3
Aspartic acid
94
Glycine
From these results, what would you conclude about the functional
significance of this glutamic acid within the protein?
MAKE CONNECTIONS The restriction site for an enzymecalled PvuI is the following sequence:5’-CGATCG-3’3’-GCTAGC-5’
Q1: Why is an insertion or a deletion in a gene more likely to alter the protein product than a substitution, such as A for C, would? Q2: Which would you expect to have more impact on an organism: a point mutation as shown here, or the insertion or deletion of a whole chromosome (discussed in Chapter 8)? Q3: Which mechanisms in a cell prevent mutations?
Chapter 56 Solutions
EBK CAMPBELL BIOLOGY
Ch. 56.1 - Explain why it is too narrow to define the...Ch. 56.1 - Identify the four main threats to biodiversity and...Ch. 56.1 - WHAT IF? Imagine two populations of a fish...Ch. 56.2 - How does the reduced genetic diversity of small...Ch. 56.2 - If there were 100 greater prairie chickens in a...Ch. 56.2 - WHAT IF? In 2005, at least ten grizzly bears in...Ch. 56.3 - Prob. 1CCCh. 56.3 - Prob. 2CCCh. 56.3 - Prob. 3CCCh. 56.4 - How can the addition of excess mineral nutrients...
Ch. 56.4 - Prob. 2CCCh. 56.4 - MAKE CONNECTIONS Mutagens are chemical and...Ch. 56.5 - What is meant by the term sustainable development?Ch. 56.5 - Prob. 2CCCh. 56.5 - WHAT IF? Suppose a new fishery is discovered, and...Ch. 56 - Give at least three examples of key ecosystem...Ch. 56 - Why is the minimum viable population size smaller...Ch. 56 - Prob. 56.3CRCh. 56 - Prob. 56.4CRCh. 56 - Prob. 56.5CRCh. 56 - One characteristic that distinguishes a population...Ch. 56 - The main cause of the increase in the amount of...Ch. 56 - Prob. 3TYUCh. 56 - Which of the following is a consequence of...Ch. 56 - Which of the following strategies would most...Ch. 56 - Of the following statements about protected areas...Ch. 56 - Prob. 7TYUCh. 56 - Prob. 8TYUCh. 56 - Prob. 9TYUCh. 56 - WRITE ABOUT A THEME: INTERACTIONS One factor...Ch. 56 - Prob. 11TYU
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- WHAT IF? DRAW IT The template strand of a geneincludes this sequence:3¿-TACTTGTCCGATATC-5¿. It is mutated to3¿-TACTTGTCCAATATC-5¿. For both wild-type and mutantsequences, draw the double-stranded DNA, the resultingmRNA, and the amino acid sequence each encodes. Whatis the effect of the mutation on the amino acid sequence?arrow_forwardQ9. Based on the screenshot shown in Figure 22, which reading frame contains the amino acid sequence for the second coding exon of tra-RA?arrow_forwardQ4) What are the 3 main components required for cloning? Q5) What is 16S rDNA, and why would cloning it be useful? Q6) How big is the 16S rDNA ‘amplicon’ we would be cloning?arrow_forward
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