LOOSE LEAF TEXT W/ MODIFIED MAST MICRO
5th Edition
ISBN: 9780134561837
Author: BAUMAN
Publisher: PEARSON
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Chapter 6, Problem 10CT
Starting with 10 bacterial cells per milliliter in a sufficient amount of complete culture medium with a 1-hour
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Given a log phase bacterial culture with 1 x 10^6 cells per ml and a generation time of 30 minutes how long does it take the culture to reach a density of 6.4 x 10^7 cells per ml?
Why would cells that are vigorously growing when inoculated into fresh culture medium have a shorter lag phase than those that have been stored in a refrigerator?
Assume an inoculum with a cell density of 108 cells per mL. The entire generation time takes 30 minutes. How many hours would it take to grow a culture to 108/mL if you started with a 10–2 dilution?
helpful formula:
g (generation time) = 0.301 (time)/ log x – log xo
Chapter 6 Solutions
LOOSE LEAF TEXT W/ MODIFIED MAST MICRO
Ch. 6 - Prob. 1MCCh. 6 - In the laboratory, a sterile inoculating loop is...Ch. 6 - Superoxide dismutase _____________. a. causes...Ch. 6 - The most reactive of the four toxic forms of...Ch. 6 - Microaerophiles that grow best with a high...Ch. 6 - Prob. 6MCCh. 6 - Organisms that preferentially may thrive in icy...Ch. 6 - Prob. 8MCCh. 6 - Prob. 9MCCh. 6 - In a defined medium, ______________. a. the exact...
Ch. 6 - Prob. 11MCCh. 6 - Prob. 12MCCh. 6 - Prob. 13MCCh. 6 - Lyophilization can be described as ___________. a....Ch. 6 - Prob. 15MCCh. 6 - Prob. 1FIBCh. 6 - Prob. 2FIBCh. 6 - Prob. 3FIBCh. 6 - Prob. 4FIBCh. 6 - Prob. 5FIBCh. 6 - Prob. 6FIBCh. 6 - Prob. 7FIBCh. 6 - Prob. 8FIBCh. 6 - Prob. 9FIBCh. 6 - Prob. 10FIBCh. 6 - Prob. 11FIBCh. 6 - Prob. 1VICh. 6 - Prob. 2VICh. 6 - High temperature affects the shape of particular...Ch. 6 - Support or refute the following statement:...Ch. 6 - Prob. 3SACh. 6 - Why must media, vessels, and instruments be...Ch. 6 - Prob. 5SACh. 6 - Prob. 6SACh. 6 - Draw and label the four distinct phases of a...Ch. 6 - Prob. 8SACh. 6 - Prob. 9SACh. 6 - Prob. 10SACh. 6 - Explain the differences among photoautotrophs,...Ch. 6 - Prob. 12SACh. 6 - How does a chemostat maintain a constant...Ch. 6 - A scientist describes an organism as a...Ch. 6 - Pasteurization is a technique that uses...Ch. 6 - Prob. 3CTCh. 6 - Some organisms require riboflavin (vitamin B2) to...Ch. 6 - A scientist inoculates a bacterium into a complex...Ch. 6 - How can regions within biofilms differ in their...Ch. 6 - A scientific article describes a bacterium as an...Ch. 6 - Prob. 8CTCh. 6 - Prob. 9CTCh. 6 - Starting with 10 bacterial cells per milliliter in...Ch. 6 - Prob. 11CTCh. 6 - Prob. 12CTCh. 6 - The filamentous bacterium Beggiatoa gets its...Ch. 6 - Prob. 14CTCh. 6 - Examine the graph in Figure 6.3. Note that the...Ch. 6 - Prob. 16CTCh. 6 - Using the terms in Figure 6.8a, describe the...Ch. 6 - Prob. 18CTCh. 6 - Examine the ingredients of MacConkey agar as...Ch. 6 - Prob. 20CTCh. 6 - Using as many of the following terms as...Ch. 6 - Prob. 22CTCh. 6 - Viable plate counts are used to estimate...Ch. 6 - Using the following terms, fill in the following...Ch. 6 - Prob. 1TMWCh. 6 - Why do clinical laboratory scientists keep many...Ch. 6 - Prob. 3TMWCh. 6 - Culturing Microorganisms One of Kochs postulates...Ch. 6 - Clinical Case Study Boils in the Locker Room For...
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- Assume you have a stock culture at 5 x 109 cells/mL and you wish to inoculate 1 liter of fresh medium so that in 15 hours the cell density will be 2 x 108/mL. Assume a generation time of 3.5 hours. What should be the dilution?arrow_forwardHow long does it take for a bacterial culture with a generation time of 12 minutes to increase from 400 cells up to 1600 cells/mL?arrow_forwardWhat would the final concentration of a bacteria culture be if 2.7 x 106 cells/ml were diluted 8.2 x 10-2? What would the initial concentration of a bacteria culture be if the final concentration was 3.7 x 102 cells/ml and the total dilution was 4.6 x 10-4?arrow_forward
- In an experiment to calculate the decimal reduction time for an Escherichia coli culture, viable cells were exposed to a constant temperature of 80°C for a set amount of time. After exposure, the remaining number of surviving cells were counted. Based on Table 1, what is the decimal reduction time?Table 1. Decimal Reduction Time for E. coli Heated to 80°C Total time of exposure (minutes): Number of Microbial Cells Present: 0 100 1 80 3 50 4 42 6.5 26 13 10 21 0arrow_forwardCalculate the growth rate and generation time of a culture that increases in the exponential phase from 5 x 102 to 1 x 108 cells in 12 hours.arrow_forwardWhat is the advantage of the plating method over an electronic cell counting method in counting cells? Why does turbidity lose reliability at high cell concentrations when the culture reaches the stationary phase?arrow_forward
- Using one of the graphs obtained with the medium II, name the different phases of the bacterial growth and indicate the starting and ending time points of these phases as well as the physiological characteristics of the bacteria during each phasearrow_forwardIf a culture had 4 cells to begin with and has a generation time of 60 minutes, how long would it take to get 1,048,576 cells?arrow_forwardA serial dilution of a bacterial culture yields the following number of colonies. Which plate(s) should be used to determine the original cell density? Plate A Plate B Plate C Plate D Plate E Dilution Factor 10-5 10-6 10-7 10-8 10-9 # of Colonies Too many to count 850 456 80 14 Group of answer choices All of these choices A & B D & E E None of these choices A B & C D B C & D Carrow_forward
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