Biology: Concepts and Investigations
5th Edition
ISBN: 9781260542202
Author: Marielle Hoefnagels
Publisher: Mcgraw-hill Higher Education (us)
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Chapter 6, Problem 10WIO
Explain the fact that species as diverse as humans and yeasts use the same biochemical pathways to extract energy from nutrient molecules.
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A “pulse-chase” experiment using 14C-labeled carbon sources is carried out on a yeast extract maintained under strictly anaerobic conditions to produce ethanol. The experiment consists of incubating a small amount of14C-labeled substrate (the pulse) with the yeast extract just long enough for each intermediate in the fermentation pathway to become labeled. The label is then “chased” through the pathway by the addition of excess unlabeled glucose. The chase effectively prevents any further entry of labeled glucose into the pathway.(a) If [1-14C]glucose (glucose labeled at C-1 with 14C) is used as a substrate, what is the location of 14C in the product ethanol? Explain.(b) Where would 14C have to be located in the starting glucose to ensure that all the 14C activity is liberated as 14CO2 during fermentation to ethanol? Explain.
Use drawings, flow charts, or a table to compare and contrast the energy inputs and outputs during each phase of aerobic respiration vs. fermentation. Be sure to directly discuss inputs and outputs of BOTH processes. Include all phosphorylated compounds and high-energy electron carriers, and briefly EXPLAIN HOW these are produced at each stage of respiration. WHERE does each stage happen? Explain HOW energy from high energy electron carriers is converted to ATP during respiration in mitochondria or aerobic bacteria.
Look at the cellular respiration reaction. What reactants and/or catalysts (enzymes) does the yeast supply? What does the juice supply?
What is the product of respiration that we need? In other words, why do organisms need cellular respiration?
Propose an alternative experiment to test rates of cellular respiration besides measuring acid production (hint: look at the reaction equation).
Do some reading and discuss some applications of fermentation in biotechnology, food production, or medicine.
Chapter 6 Solutions
Biology: Concepts and Investigations
Ch. 6.1 - Why do all organisms need ATP?Ch. 6.1 - What are the three general ways to generate ATP...Ch. 6.1 - Prob. 3MCCh. 6.1 - Prob. 4MCCh. 6.2 - Prob. 1MCCh. 6.2 - What occurs in each of the three stages of...Ch. 6.3 - Prob. 1MCCh. 6.3 - Prob. 2MCCh. 6.4 - Prob. 1MCCh. 6.4 - Prob. 2MC
Ch. 6.4 - Prob. 3MCCh. 6.5 - Prob. 1MCCh. 6.5 - Prob. 2MCCh. 6.5 - Prob. 3MCCh. 6.5 - Prob. 4MCCh. 6.6 - Prob. 1MCCh. 6.6 - How does the actual ATP yield compare to the...Ch. 6.7 - Prob. 1MCCh. 6.7 - Prob. 2MCCh. 6.8 - Prob. 1MCCh. 6.8 - Prob. 2MCCh. 6.8 - Prob. 3MCCh. 6.9 - Prob. 1MCCh. 6.9 - Why must the first metabolic pathways have been...Ch. 6.9 - What is the evidence that photosynthesis may have...Ch. 6.10 - What hypothesis were the researchers testing, and...Ch. 6.10 - Prob. 2MCCh. 6 - Which of the following best describes aerobic...Ch. 6 - Which stage in cellular respiration produces the...Ch. 6 - What is the role of ATP synthase? a. It uses ATP...Ch. 6 - Prob. 4MCQCh. 6 - Prob. 5MCQCh. 6 - What is endosymbiosis? a. A type of fermentation...Ch. 6 - Respiration contains the Latin word root spiro,...Ch. 6 - All steps of cellular respiration are closely...Ch. 6 - How might a mitochondrion's double membrane make...Ch. 6 - Health-food stores sell a product called pyruvate...Ch. 6 - At what point does O2 enter the energy pathways of...Ch. 6 - Describe the energy pathways that are available...Ch. 6 - Prob. 7WIOCh. 6 - Describe how aerobic respiration occurs in...Ch. 6 - Prob. 9WIOCh. 6 - Explain the fact that species as diverse as humans...Ch. 6 - Prob. 11WIOCh. 6 - Prob. 1PITCh. 6 - Prob. 2PITCh. 6 - Prob. 3PITCh. 6 - Where would fermentation, anaerobic respiration,...
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