Chapter 6, Problem 12P

(a)

Summary Introduction

To determine: The total enzyme [E_t] that was used in experiment.

Introduction:

Enzyme is defined as a biological catalyst that speeds up the rate of chemical reaction. All biological reactions are catalyzed by enzymes. Every enzyme has active site at which a substrate binds and the substrate is then converted into product.

(b)

Summary Introduction

To determine: The value of [X]used in experiment.

Introduction:

Enzyme contains an active site which is a region of enzyme where substrate binds. After binding of enzyme with substrate, an enzyme-substrate complex is formed and initiates the rate of chemical reaction.

(c)

Summary Introduction

To determine: The value of [X]in the presence of inhibitor

Introduction:

Inhibitors are the chemical molecules that inhibit the activity of enzyme by binding either at active site of enzyme or region other than the active site. It prevents the binding of substrate by changing the shape and conformation of enzyme.

(d)

Summary Introduction

To explain: This enzyme is evolved to achieve catalytic perfection.

Introduction:

Enzyme kinetics is defined as the study of chemical reactions which is catalyzed by enzyme. It indicates a rate of reaction as a function of substrate concentration with $V\max$ and $Km$ of enzyme.