Microbiology: An Introduction Plus Mastering Microbiology with Pearson eText -- Access Card Package (13th Edition) (What's New in Microbiology)
13th Edition
ISBN: 9780134688640
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Chapter 6, Problem 3A
Most laboratory media contain a fermentable carbohydrate and peptone because the majority of bacteria require carbon, nitrogen, and energy sources in these forms. How are these three needs met by glucose-minimal salts medium? (Hint: See Table 6.2.)
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Menaquinones is produced by Flavobacterium meningosepticum via submerged fermentation with 60% working volume in 30-L fermentation. To obtain the crude menaquinones, the fermentation medium is centrifuged at 12,000 rpm for 15 minutes. The vacuum-freeze dried particles are crushed and collected. After that the samples are treated with methanol for 20 minutes. To remove polar lipid compounds from bacterial cells, HZ816 macroporous adsorption resin column is used with methanol-dichloromethane (l:1) as mobile phase and flow rate of 0.75 mL/min. Rotary evaporation at 500 C is carried out to concentrate the eluent. After that, the concentrated sample is purified with Bio-BeadsTM S-X3 to remove components without UV absorption. To obtain the homolog purified menaquinones, RP-C18 column is used. Recrystallization is carried out to obtain pure crystalized menaquinones.
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Indicate how to obtain the sample in the methanol solvent.
Three different chromatography are used in…
Sydney Brenner isolated Salmonella typhimurium mutants that were
implicated in the biosynthesis of tryptophan and would not grow on
minimal medium. When these bacterial mutants were tested on minimal
medium to which one of four compounds (indole glycerol phosphate,
indole, anthranilic acid, and tryptophan) had been added, the growth
responses shown in the following table were obtained.
Mutant
Minimal
medium
Anthranilic Indole glycerol
acid
Indole Tryptoph
phosphate
trp-1
trp-2
trp-3
trp-4
trp-6
trp-7
trp-8
trp-9
trp-10
trp-11 -
Give the order of indole glycerol phosphate, indole, anthranilic acid, and
tryptophan in a biochemical pathway leading to the synthesis of
tryptophan. Indicate which step in the pathway is affected by each of the
mutations.
+ 1 + 1 II
explain why there is little to no growth of these organisms in the minimal salt media with glucose compared to the complex media. (HINT: these organisms are chemoheterotrophs and need an organic source of broth carbon and energy)...
Chapter 6 Solutions
Microbiology: An Introduction Plus Mastering Microbiology with Pearson eText -- Access Card Package (13th Edition) (What's New in Microbiology)
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