Concept explainers
A.
To determine: If DNA polymerase was missing during
Concept introduction: DNA consists of genetic instructions in the form of gene sequences. Replication of DNA is semiconservative. The parental strand acts as a template for the formation of new daughter strand. This process is performed at the beginning of cell division so that each daughter cell inherits an identical DNA copy. DNA replication proceeds with a three step process and requires template DNA strand, primers, DNA
B.
To determine: If DNA ligase was missing during DNA replication process.
Concept introduction: DNA consists of genetic instructions in the form of gene sequences. Replication of DNA is semiconservative. The parental strand acts as a template for the formation of new daughter strand. This process is performed at the beginning of cell division so that each daughter cell inherits an identical DNA copy. DNA replication proceeds with a three step process and requires template DNA strand, primers, DNA nucleotides, and enzymes- DNA polymerase, primase, helicase, ligase, and topoisomerase.
C.
To determine: If sliding clamp for DNA polymerase was missing during DNA replication process.
Concept introduction: DNA consists of genetic instructions in the form of gene sequences. Replication of DNA is semiconservative. The parental strand acts as a template for the formation of new daughter strand. This process is performed at the beginning of cell division so that each daughter cell inherits an identical DNA copy. DNA replication proceeds with a three step process and requires template DNA strand, primers, DNA nucleotides, and enzymes- DNA polymerase, primase, helicase, ligase, and topoisomerase.
D.
To determine: If nuclease that removes primers was missing during DNA replication process.
Concept introduction: DNA consists of genetic instructions in the form of gene sequences. Replication of DNA is semiconservative. The parental strand acts as a template for the formation of new daughter strand. This process is performed at the beginning of cell division so that each daughter cell inherits an identical DNA copy. DNA replication proceeds with a three step process and requires template DNA strand, primers, DNA nucleotides, and enzymes- DNA polymerase, primase, helicase, ligase, and topoisomerase.
E.
To determine: If DNA helicase was missing during DNA replication process.
Concept introduction: DNA consists of genetic instructions in the form of gene sequences. Replication of DNA is semiconservative. The parental strand acts as a template for the formation of new daughter strand. This process is performed at the beginning of cell division so that each daughter cell inherits an identical DNA copy. DNA replication proceeds with a three step process and requires template DNA strand, primers, DNA nucleotides, and enzymes- DNA polymerase, primase, helicase, ligase, and topoisomerase.
F.
To determine: If primase was missing during DNA replication process.
Concept introduction: DNA consists of genetic instructions in the form of gene sequences. Replication of DNA is semiconservative. The parental strand acts as a template for the formation of new daughter strand. This process is performed at the beginning of cell division so that each daughter cell inherits an identical DNA copy. DNA replication proceeds with a three step process and requires template DNA strand, primers, DNA nucleotides, and enzymes- DNA polymerase, primase, helicase, ligase, and topoisomerase.
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