Concept explainers
MATHEMATICAL For the following aspartase reaction (see Question 28) in the presence of the inhibitor hydroxymethylaspartate, determine
Interpretation:
The
Concept introduction:
In an enzymatic reaction, the inhibitors are the molecules that bind with the enzymes and block their activity. Enzymatic inhibition can be of many types. If a substrate and an inhibitor compete with each other to bind with the enzyme and both have similar structures, then the inhibition is known as competitive. On the other hand, if an inhibitor does not compete with the substrate and both of them can bind to an enzyme at their respective specific sites, then it is known as non-competitive inhibition.
Answer to Problem 61RE
Plot the reciprocal substrate concentration on the x-axis and two reaction velocities (both in the presence and absence of inhibitors) on the y-axis.
Before drawing the plot, the data needs to be modified so that it can be aligned in the Lineweaver–Burk plot. The modification of the data is as follows:
The Lineweaver–Burk plot from above data is:
From this plot
In an uninhibited reaction, the
Therefore, the value of
Therefore, the value of
By comparing the Lineweaver–Burk plot of both reactions, it can be said that the inhibition is competitive in nature
Explanation of Solution
Given information:
In competitive inhibition, the substrate and the inhibitor have a similar structure. Hence, like the substrate, the inhibitor has also got nearly same affinity toward the active site of an enzyme and is able to bind with the enzyme. As a result, the enzyme’s affinity towards the substrate changes (it inhibits the substrate to bind with the enzyme). In other words, the
Also, in Lineweaver–Burk plot the
The reaction (without inhibitor) has a low value of
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Chapter 6 Solutions
Biochemistry
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- BiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage Learning