Concept explainers
PROCESS OF SCIENCE In an experiment, you create two groups of liposomes in a solution containing 0.1M NaCl—one made from red blood cell membranes and the other from frog egg cell membranes. When the liposomes are placed in water, those with red blood cell membranes burst more rapidly than those made from egg membranes. Evaluate each of the following statements and identify those that could explain these results.
• T/F The red blood cell liposomes are more hypertonic relative to water than the frog egg liposomes.
• T/F The red blood cell liposomes are more hypotonic relative to water than the frog egg liposomes.
• T/F The red blood cell liposomes contain more aquaporins than the frog egg liposomes.
• T/F The frog egg liposomes contain ion channels, which are not present in the red blood cell liposomes.
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Chapter 6 Solutions
Biological Science (7th Edition)
- a. You selectively label phospholipids with a fluorescent dye and perform the FRAP assay. You detect a single fluorescence molecule move 100 micrometre into the photobleached area in 2.5 seconds. What is the diffusivity constant for this phospholipid? b. Increasing the ratio of unsaturated to saturated phospholipids in a biological membrane increases membrane fluidity. Describe how the FRAP photobleaching and recovery curves would differ between membranes with 63% and 41% unsaturated phospholipids. c. Draw the FRAP photobleaching and recovery curves for your answer in b. d. What does the “immobile fraction” in a FRAP experiment suggest (see diagram)?arrow_forwardYou are viewing two samples of mammalian red blood cells (erythrocytes) under a microscope. One first sample is made up of cells with a typical round shape. Most of the cells in the second sample had burst open. •Describe the properties of the solution that was used to prepare the second sample. • Identify one type of solute that can move through simple diffusion and does not affect osmotic pressure or require a membrane protein to cross a plasma membrane.arrow_forwardName the three major assumptions made by the "Cell theory". (i) The lipid membrane is composed of lipid molecules. Explain the principle of membrane formation highlighting the role of the physical properties of the lipids. (ii) Comparing dimensions and length scales is often a first step in an analysis. Give an approximate value for the thickness of a lipid bilayer and the linear length of a helical turn of a DNA double helix. A technician wants to amplify DNA from a patient sample. However, the lab is not equipped with a thermocycler. (i) (ii) Name two methods for DNA amplification that can be operated at constant temperature and give their acronyms. Explain these two methods in detail using a schematic and name all necessary components that are required to perform the amplification. Describe the main function of the middle ear. Highlight the role of the ossicles and the tympanic membrane.arrow_forward
- - The classic demonstration that cell plasma membranes are com- posed of bilayers depends on the following kinds of data: • The membrane lipids from 4.74 x 10° erythrocytes will form a monolayer of area 0.89 m² when spread on a water surface. • The surface of one erythrocyte is approximately 100 µm? in area. Show that these data can be accounted for only if the erythrocyte membrane is a bilayer.arrow_forward;, Consider a spherical cell at body temperature (37°C) that is 10 µm in radius. The concentration of ions inside and outside the cell are given below. Sodium and potassium are the only permeant ions, and the membrane contains proteins that pump in 3 sodium ions for every 2 potassium ions pumped out. [Na*] = ? [K*] = 119mM [Ci] = 8mM [A] = 121mM [Na*] = 120mM [K*] = ? [Cr] = 125MM [glu] = ? (a) Suppose the cell is in osmotic equilibrium, find the 3 unknown concentrations. (b) Suppose the membrane conductance to sodium is 5% that to potassium, find the resting membrane potential via circuit analysis. (c) What would the resting potential be if the membrane were instead permeable only to chloride ions? (d) Suppose the cell was suddenly placed in distilled water, what would happen to it? And, how much external pressure (positive or negative) would be needed to prevent this from happening?arrow_forwardMs. Sassa, a biology professor, wanted to demonstrate to her students the applicability of a dialyzing membrane (DM) as a model for the cell membrane by enclosing an aqueous solution in a DM bag and immersing in a beaker containing a different solution. Substances available which are permeable to the DM include 0.02 M NaCl, 0.03 M glucose, and 0.01 M glucose. The only substance available which is completely impermeable to the DM is the 0.01 M lactose. Using the substances given and materials such as a beaker, stirring rod, and string, draw ONLY ONE set-up that can be demonstrated by Ms. Sassa that will satisfy ALL of the following conditions: a. No solute will exhibit a net diffusion out of the cell. b. Glucose will exhibit a net diffusion into the cell. c. NaCl will exhibit a zero net movement. d. No net movement of lactose from the inside to outside of the cell. Make sure to label properly the substance inside the beaker and inside the DM bag.arrow_forward
- Shown below are cells (colored) that were recently placed into a beaker containing a clear solution For each scenario, indicate whether movement of the molecule into the cell will occur using facilitated diffusion or active transport. А. B. 125mM 20mM fructose glucose 85MM 35mM fructose glucose OA= facilitated diffusion; B= facilitated diffusion OA= active transport; B= active transport O A= active transport; B= facilitated diffusion A= facilitated diffusion; B= active transportarrow_forwardb) If you soak an animal cell that is permeable to both water and glucose in either 5.5% glucose or 0.9% NaCl (both isosmotic solutions), the cell exposed to 5.5% glucose will gain water, while the cell exposed to 0.9% NaCl will not gain water. Predict why this is the case.arrow_forwardMembrane lipid molecules exchange places with their lipid neighbors every 10–7 second. A lipid molecule diffuses from one end of a 2-μm-long bacterial cell to the other in about 1 second. Are these two numbers in agreement (assume that the diameter of a lipid head group is about 0.5 nm)? If not, can you think of a reason for the difference?arrow_forward
- uring dialysis, a semi-permeable membrane with a molecular weight cutoff of 10,000 is used. Select the true statements from the list below (more than one answer could apply) Group of answer choices A monoclonal antibody with a 150 kDa MW is retained and does not diffuse across the membrane A small enzyme with 20 kDa MW diffuses readily across the membrane Buffer salts with molecular weights ranging between 100 and 500 Da diffuse across the membrane until equilibrium (equal concentration) is reached on both sides of the membrane None of the above statements are correct Please answer asaparrow_forwardCalculate the number of cells in the body of an average 68-kg (150-lb) adult. (This will only be accurate to about 1 part in 10 but should give you an idea how scientists estimate this commonly quoted number.) Assume all cells are spheres 20 m in diameter. The volume of a sphere can be determined by the equation v=4/33. (Hint: We know that about two thirds of the water in the body is intracellular and the density of cells is nearly 1 g/mL. The proportion of the mass made up of water is about 60%.)arrow_forwarddentify the structure labeled A (thick line) I Choose | (Choose dentify the structure labeledB Cell membrane dentify the structure labeledC Cell wall Nucleus Jentify the structure labeled D (thin line belween the whiteeand purple) yloplasmarrow_forward
- Human Physiology: From Cells to Systems (MindTap ...BiologyISBN:9781285866932Author:Lauralee SherwoodPublisher:Cengage Learning
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