Nester's Microbiology: A Human Perspective
9th Edition
ISBN: 9781259709999
Author: Denise G. Anderson Lecturer, Sarah Salm, Deborah Allen
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 7, Problem 10MC
Which of the following statements is false?
a) A derivative of lactose serves as an inducer of the lac operon.
b) Signal transduction provides a mechanism for a cell to sense the conditions of its external environment.
c) Quorum sensing allows bacterial cells to sense the density of like cells.
d) An example of a two-component regulatory system is the lactose operon, which is controlled by a repressor and an activator.
e) An ORF is a stretch of DNA that may encode a protein.
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In the absence of tryptophan and the promoter sequence was mutated such that the RNA polymerase can no longer bind, the structural genes will:[ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"]
In the presence of tryptophan and the promoter sequence was mutated such that the RNA polymerase can no longer bind, the structural genes will:[ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"]
What type of mutation in an operon is most likely to affect the synthesis of more than one protein?
In the absence of lactose and the lacY structural gene contains a missense point mutation, the structural genes will: [ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"]
In the presence of lactose and the lacY structural gene contains a missense point mutation, the structural genes will: [ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"]
In the absence of lactose and the lacA structural gene contains a frameshift deletion mutation, the structural genes will: [ Select ] ["No longer be expressed", "Be constantly expressed but the protein will be truncated and possibly non-functional", "Be constantly expressed but the protein will be longer and possibly non-functional"]
In the presence of lactose and the lacA structural gene contains a frameshift deletion mutation, the structural genes will: [ Select ] ["No longer be expressed", "Be constantly expressed but the protein will be truncated and possibly…
Chapter 7 Solutions
Nester's Microbiology: A Human Perspective
Ch. 7 - Explain what semiconservative means with respect...Ch. 7 -
2. What is an origin of replication?
Ch. 7 - Why are primers required in DNA replication but...Ch. 7 -
4. What is polycistronic mRNA?
Ch. 7 -
5. Explain why knowing the orientation of a...Ch. 7 - What is the function of a sigma factor?Ch. 7 - Prob. 7SACh. 7 - Explain how some bacteria sense the density of...Ch. 7 -
9. Compare and contrast regulation by a repressor...Ch. 7 - Prob. 10SA
Ch. 7 - All of the following are involved in transcription...Ch. 7 -
2. All of the following are involved in DNA...Ch. 7 - All of the following are directly involved in...Ch. 7 - Using the DNA strand shown here as a template,...Ch. 7 -
5. A ribosome binds to the following mRNA at the...Ch. 7 - Which of the following statements about gene...Ch. 7 -
7. An enzyme used to synthesize the amino acid...Ch. 7 -
8. Under which of the following conditions will...Ch. 7 -
9. All of the following are characteristics of...Ch. 7 - Which of the following statements is false? a) A...Ch. 7 - A graduate student is trying to identity the gene...Ch. 7 - To isolate eukaryotic mRNA from cell lysates, a...Ch. 7 - Prob. 1CTCh. 7 -
2. In a variation of the experiment in the...
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- What is the difference between positive regulation of the lac operon and negative regulation of the lac operon?arrow_forwardLet’s suppose you have isolated a mutant strain of E. coli in which the lac operon is constitutively expressed. In other words, the operon is turned on in the presence or absence of lactose. One possibility is that the mutation may block the transcription of the lacI gene, thereby preventing the synthesis of lac repressor. A second possibility is that the mutation could alter the sequence of the lac operon in a way that prevents the repressor protein from binding to the operator. How would you distinguish between these two possibilities?arrow_forwardwhat is the lac operon ? what happens in terms of the lac operon when lactose is absent from the growth medium?!arrow_forward
- What would happen if the operator sequence of the trp operon contained a mutation that prevented the repressor protein from binding to the operator? (Explain what would happen in both the presence and absence of tryptophan)arrow_forwardLet’s suppose you have isolated a mutant strain of E. coli in which the lac operon is constitutively expressed. In other words, the operon is turned on in the presence or absence of lactose. One possibility is that the mutation mayblock the transcription of the lacI gene, thereby preventing the synthesis of lac repressor. A second possibility is that the mutation could alter the sequence of the lac operator in a way that prevents lac repressor from binding to the operator. How would you distinguish between these two possibilities?arrow_forwardWhat is lac operon?arrow_forward
- When lactose is first introduced into the medium, the lac operon is not induced and thus permease isn't induced. How is it possible for lactose to enter the cell before the lac operon is induced? While the lac operon is not induced yet, lactose has many different ways to get into the cells without the need of permease. While the lac operon is not induced yet, lactose breaks down outside the cell into smaller components in order to get into the cell and then the components reassemble into lactose again inside the cell so that the induction can occur. While the lac operon is not induced yet, there is always a very high level of permease and beta galactosidase in the cell. While the lac operon is not induced yet, there is full level expression most of the time which allows a sufficient amount of permease to be expressed. This allows lactose to enter the cell. While the lac operon is not induced yet, there is low level expression most of the time which…arrow_forwardIn the presence of tryptophan and the promoter sequence was mutated such that the RNA polymerase can no longer bind, the structural genes will:[ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"] In the absence of tryptophan and the trpR repressor gene is mutated such that the repressor protein product can no longer bind to the operator, the structural genes will:[ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"] In the presence of tryptophan and the trpR repressor gene is mutated such that the repressor protein product can no longer bind to the operator, the structural genes will:[ Select ] ["No longer be expressed", "Be constantly expressed", "Be constantly expressed"]arrow_forwardWhat is the function of the Z gene of the Lac operon?arrow_forward
- In the presence of lactose and the promoter sequence was mutated such that the RNA polymerase can no longer bind, the structural genes will:[ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"] In the absence of lactose and the lacI repressor gene is mutated such that the repressor protein product can no longer bind to the operator, the structural genes will: [ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"] In the presence of lactose and the lacI repressor gene is mutated such that the repressor protein product can no longer bind to the operator, the structural genes will: [ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"]arrow_forwardAccording to the operon model, for the synthesis of an inducible enzyme to occur, the end-product must not be in excess an inducer must bind to the enzyme an inducer must bind to the repressor repressor must bind to the operator repressor must not be synthesizedarrow_forwardA mutation occurs in the 5′ UTR of the trp operon that reduces the ability of region 2 to pair with region 3. What will the effect of this mutation be when the tryptophan level is high? When the tryptophan level is low?arrow_forward
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