Nester's Microbiology: A Human Perspective
9th Edition
ISBN: 9781259709999
Author: Denise G. Anderson Lecturer, Sarah Salm, Deborah Allen
Publisher: McGraw-Hill Education
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Chapter 7, Problem 2A
To isolate eukaryotic mRNA from cell lysates, a researcher has purchased magnetic beads that have short
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How is it possible that a given mRNA in a cell is found throughout the cytoplasm but the protein that it encodes is only found in a few specific regions of the cytoplasm? Cite a few different possibilities.
A eukaryotic cell carrying out transcription and RNA processing is incubated with 32P-labeled ATP. Where will the radioactive isotope appear in mature mRNA if the ATP is labeled at the (a) α position, (b) β position, and (c) γ position?
When a eukaryotic gene is cut out of genomic DNA, geneticists have discovered that enabling the strands to hydrogen bond allows them to hybridize one of the gene's strands to the mRNA for that gene.
How can you tell whether this gene undergoes alternative splicing by comparing the mRNAs that result?
Chapter 7 Solutions
Nester's Microbiology: A Human Perspective
Ch. 7 - Explain what semiconservative means with respect...Ch. 7 -
2. What is an origin of replication?
Ch. 7 - Why are primers required in DNA replication but...Ch. 7 -
4. What is polycistronic mRNA?
Ch. 7 -
5. Explain why knowing the orientation of a...Ch. 7 - What is the function of a sigma factor?Ch. 7 - Prob. 7SACh. 7 - Explain how some bacteria sense the density of...Ch. 7 -
9. Compare and contrast regulation by a repressor...Ch. 7 - Prob. 10SA
Ch. 7 - All of the following are involved in transcription...Ch. 7 -
2. All of the following are involved in DNA...Ch. 7 - All of the following are directly involved in...Ch. 7 - Using the DNA strand shown here as a template,...Ch. 7 -
5. A ribosome binds to the following mRNA at the...Ch. 7 - Which of the following statements about gene...Ch. 7 -
7. An enzyme used to synthesize the amino acid...Ch. 7 -
8. Under which of the following conditions will...Ch. 7 -
9. All of the following are characteristics of...Ch. 7 - Which of the following statements is false? a) A...Ch. 7 - A graduate student is trying to identity the gene...Ch. 7 - To isolate eukaryotic mRNA from cell lysates, a...Ch. 7 - Prob. 1CTCh. 7 -
2. In a variation of the experiment in the...
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- Compare bacterial and eukaryotic mRNAs, and explain the functional significance of their structural differences.arrow_forwardWhat are the three modifications made to pre-mRNA molecules before they become mature mRNAs, are transported from the nucleus to the cytoplasm, and become ready to be used in protein synthesis? What is the function of each modification?arrow_forwardHere is a eukaryotic gene. The numbers given are base pairs of exon and intron. How long in bases will the pre mRNA transcript be? Explain briefly. What is the maximum number of amino acids that could make up the protein product from the final mRNA? Explain briefly.arrow_forward
- Consider the following mRNA base sequence 5' CUG-CAC 3' (a) What dipeptide is coded for by this mRNA? (b) What dipeptide is formed if a mutation converts CUG to CUU? (c) What dipeptide is formed if a mutation converts CAC to CGC? (d) What dipeptide is formed if a mutation converts CUG to CUU and CAC to CGC?arrow_forwardWhen scientists cut a eukaryotic gene from genomic DNA, they discovered that enabling the strands to hydrogen bond allows them to hybridize one of the gene's strands to the gene's mRNA. How can you tell whether this gene undergoes alternative splicing by comparing the various mRNAs that result?arrow_forwardWhat polypeptide product would you expect from apoly-G mRNA that is 30 nucleotides long?arrow_forward
- What is a polycistronic mRNA? How are polycistronic mRNAs formed?arrow_forwardThe asterisk (*) in the diagram below indicates a single base mutation in the 5' splice site of the second intron of a eukaryotic gene. Due to this mutation, the second intron is now not ‘spliced out’ during the splicing process. What are the most likely consequences of this mutation with respect to the size of the pre-mRNA and the size of the mature mRNA? a. The pre-mRNA will be longer and the mature mRNA will be longer. b. The pre-mRNA will be longer and the size of the mature mRNA will not be affected c. The size of the pre-mRNA will not be affected and the mature mRNA will be longer d. The size of the pre-mRNA will not be affected and the size of the mature mRNA will not be affectedarrow_forwardWhat features do mature eukaryotic mRNA molecules have that bacterial mRNAs lack?arrow_forward
- What are the steps involved in the processing of eukaryotic pre-mRNA? Why is this process important?arrow_forwardThe following is as segment of mRNA: 5'-UCGGAAUGUGGUGGCAUACAGGCUUACAGAACUAAGUCUGAGAAU-3' A. How many amino acids long will be the protein translated from the only reading frame available in this segment? B. If a mutation changes the third letter of the stop codon in the only reading frame available in this segment, how many amino acids long will be the protein translated?arrow_forwardKnow the name of the RNA molecule that is the initial product of the transcription of a eukaryotic structural gene. Why is it called this? Relative to the gene, what sequences does this molecule contain?arrow_forward
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