MasteringMicrobiology with Pearson Etext - Valuepack Access Card - for Microbiology with Diseases by Body System
4th Edition
ISBN: 9780321943705
Author: BAUMAN
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Question
Chapter 8, Problem 10MC
Summary Introduction
Introduction:
DNA microarray is composed of single-stranded DNA molecules (ssDNA) as cDNA or genomic DNA, which are attached to the solid substrate, such as glass slide, a nylon membrane, or silicon chips. It is also called DNA chip as it contains a collection of thousands of various DNA molecules at a specific site on the array. The single array may contain DNA molecule from one or more species. The fluorescent ssDNA molecules are used as labels, which bind to their complementary sequence of DNA on the array. This technique is used to find DNA or RNA in a sample and to evaluate the expression of genes.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
A DNA fingerprint is based on _____.
a
repeating sequences found on noncoding regions
b
the presence of restriction enzymes
c
a single repeating region
d
repeating sequences found on coding regions
Which type of cells were used to extract the DNA that was sequenced? a. red blood cells c. white blood cells b. intestinal epithelium d. cheek swab What type of mutation caused Nicholas’s disease? a. frameshift c. nonsense b. missense d. insertion
The function of a restriction enzyme is to
a. prevent the movement of DNA outside the nucleus
b. separate the DNA double helix
c. cut the nucleotide sequence at a specific location in DNA
d. proofread DNA for accidental damages and corrects these errors
Chapter 8 Solutions
MasteringMicrobiology with Pearson Etext - Valuepack Access Card - for Microbiology with Diseases by Body System
Ch. 8 - Why arent the terms recombinant DNA technology...Ch. 8 - Prob. 2TMWCh. 8 - Why wasnt polymerase chain reaction (PCR)...Ch. 8 - Why dont doctors routinely insert genes into their...Ch. 8 - Prob. 5TMWCh. 8 - Which of the following statements is true...Ch. 8 - A DNA gene synthesized from an RNA template is...Ch. 8 - Prob. 3MCCh. 8 - Prob. 4MCCh. 8 - Prob. 5MC
Ch. 8 - Prob. 6MCCh. 8 - Prob. 7MCCh. 8 - Prob. 8MCCh. 8 - Prob. 9MCCh. 8 - Prob. 10MCCh. 8 - Modified True/False 1. ________ Restriction...Ch. 8 - Modified True/False 2. ________ Restriction...Ch. 8 - Prob. 3MTFCh. 8 - Prob. 4MTFCh. 8 - Prob. 5MTFCh. 8 - Label the reagents and steps of PCR on the figure...Ch. 8 - Prob. 2VICh. 8 - Describe three artificial methods of introducing...Ch. 8 - Prob. 2SACh. 8 - Prob. 3SACh. 8 - Prob. 4SACh. 8 - List three potential problems of recombinant DNA...Ch. 8 - Examine the restriction sites listed in Table 8.1...Ch. 8 - CRITICAL THINKING 2 A cancer-inducing virus,...Ch. 8 - A thermocycler uses DNA polymerase from...Ch. 8 - Prob. 4CTCh. 8 - Prob. 5CTCh. 8 - Prob. 6CTCh. 8 - Prob. 7CTCh. 8 - Prob. 8CTCh. 8 - Prob. 9CTCh. 8 - Using the following terms, fill in the following...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- What carries a gene from one organism into a bacteria cell? a. a plasmid b. an electrophoresis gel c. a restriction enzyme d. polymerase chain reactionarrow_forwardWhy is DNA ligase so important in recombinant DNA technology? a.) It causes DNA to make multiple copies of itself. b.) It joins two DNA fragments together. c.) It shapes bacterial DNA into a circular plasmid. d.) It cuts DNA into restriction fragments.arrow_forwarda) what are restriction enzymes? b) What is the main function of restriction enzymes in nature? c) Compare and contrast the these enzymes in nature and in scientific research.arrow_forward
- Restriction enzymes found in bacterial cells are ordinarily useda. during DNA replication.b. to degrade the bacterial cell’s DNA.c. to degrade viral DNA that enters the cell.d. to attach pieces of DNA together.arrow_forwardPCR is essential for Select one: A. allowing restriction enzymes to cut DNA. B. making many copies of a small amount of DNA. C. cutting DNA into many small pieces. D. creating recombinant plasmids.arrow_forwardWhy is it important to use a hyperthermophilic DNA polymerase in PCR? a) Because only hyperthermophiles have DNA polymerases. b) Because hyperthermophilic DNA polymerase is able to resist the saline reaction conditions. c) Because hyperthermophilic DNA polymerase is faster than other polymerases. d) Because hyperthermophilic DNA polymerase is able to resist denaturation at 95℃.arrow_forward
- Explain how electrophoresis separates DNA strands. a. How is a DNA fingerprinting test interpreted? b. Define plasmid and how plasmids can change a bacteria’s activity. c. How do we digest/cleave plasmids? Explain the role of a restriction enzyme. d. Define sticky end and blunt end and which one is useful in molecular biology.arrow_forwardRegarding the PCR technique, what is false?a. It can produce multiple copies of DNA.b. It is the same as DNA fingerprinting.c. It is not a time-consuming process.d. It cannot successfully copy whole genesarrow_forwardWhat is a cloning vector? A. The DNA probe used to locate a particular gene in the genome. B. An agent such as plasmid, used to transfer DNA from an in vitro solution into a living cell. C. The laboratory apparatus used to clone genes. D. An enzyme that cuts DNA into restriction fragments.arrow_forward
- Which of the following is FALSE about current Sanger dideoxy DNA sequencing procedures? a. Chain termination occurs during synthesis of a new DNA strand. b. Many steps can be automated. c. No DNA is synthesized in the procedure. d. Fluorescent molecules can be used to detect the DNA.arrow_forwardChoose the combination of answers that most accurately completes the statement.For which of the following would a nucleic acid probe not be used? a. locating a gene on a chromosome b. developing a Southern blot c. identifying a microorganism d. constructing a recombinant plasmidarrow_forwardA contig is a. a set of molecular markers used in gene mapping. b. a set of overlapping fragments that form a continuous stretch of DNA. c. a set of fragments generated by a restriction enzyme. d. a small DNA fragment used in sequencing.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Concepts of BiologyBiologyISBN:9781938168116Author:Samantha Fowler, Rebecca Roush, James WisePublisher:OpenStax College
Biology: The Dynamic Science (MindTap Course List)BiologyISBN:9781305389892Author:Peter J. Russell, Paul E. Hertz, Beverly McMillanPublisher:Cengage Learning
Concepts of Biology
Biology
ISBN:9781938168116
Author:Samantha Fowler, Rebecca Roush, James Wise
Publisher:OpenStax College
Biology: The Dynamic Science (MindTap Course List)
Biology
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Cengage Learning
Molecular Techniques: Basic Concepts; Author: Dr. A's Clinical Lab Videos;https://www.youtube.com/watch?v=7HFHZy8h6z0;License: Standard Youtube License