Campbell Essential Biology with Physiology (5th Edition)
Campbell Essential Biology with Physiology (5th Edition)
5th Edition
ISBN: 9780321967671
Author: Eric J. Simon, Jean L. Dickey, Jane B. Reece, Kelly A. Hogan
Publisher: PEARSON
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Chapter 8, Problem 13PS
Summary Introduction

To identify: Different stages of mitosis in onion root tip.

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Mitosis is a process of cell division where the parent cell divides to produce two genetically identical daughter cells. Generally, for visualization of different stages of mitosis, onion root tips are selected, as they are the rapidly growing part of the onion. To view various mitotic stages, fresh onion root tips are taken, squashed on the microscopic slide and stained with DNA stains that specifically bind to the supercoiled chromosomes. The stained structures can be visualized under light microscope.

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You prepare a slide with a thin slice of an onion root tip. You see the following view in a light microscope. Identify the stage of mitosis for each of the outlined cells, a–d
List in each blank the description of events associated with each stage of mitosis in addition to the preparatory stage Interphase. Some events and structures occur only in plant cells and some in animal cells. Mark these events in the list with an asterisk.
Draw or explain in a simple manner what needs to be done in each step of modeling mitosis. Steps/ Procedure: 1. Tie the ends of a 10-ft (3-m) piece of string together and form a circle on the table. This represents the cell membrane of a parent cell. 2. Make a smaller circle from a 5-ft (1.5-m) piece of string to create a nuclear membrane, and place it inside the cell membrane. 3. Place 1 sock of each pair into its mate and jumble the socks inside the nuclear membrane. 4. During interphase, the cell prepares for nuclear division and DNA replicates. To simulate replication, remove a mate from each pair of socks. Match sister chromatids and connect them at their centromere by tying the matching pairs together at their centers with a small piece of string. Place them back into the nuclear membrane, jumbling them up. 5. Mitotic division now begins with prophase. During this phase, DNA condenses into chromosomes made up of 2 identical sister chromatids, and the nuclear membrane breaks…
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The Cell Cycle and its Regulation; Author: Professor Dave Explains;https://www.youtube.com/watch?v=eqJqhA8HSJ0;License: Standard YouTube License, CC-BY
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