Concept explainers
To answer:
Recognition and restriction of DNA sequence by HhaI.
Introduction:
Restriction enzymes recognize and cut DNA
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- Enzymes isolated from bacteria that cut DNA at specificsequences are called ____________________.arrow_forwardIs there any situation in which DNA is made based on a RNA template? What is the enzyme involved?arrow_forwardA DNA probe with sequence TCAGGCTTCAG would bind most strongly to which of the following DNA fragments? a. AGTCCGAAGTC c. GACTTCGGACT b. TCAGGCTTCAG d. UGAGGCUUGAGarrow_forward
- The following DNAs were incubated with E. coli DNA Polymerase I and dNTPs. Write the products expected in each reaction. Indicate the activity used in each reaction.arrow_forwardA microbiologist discovers a new type II restriction endonuclease. When DNA is digested by this enzyme, fragments that average 1,048,500 bp in length are produced. What is the most likely number of base pairs in the recognition sequence of this enzyme?arrow_forwardWithin the template strand of the DNA in a gene, the sequence TAT is changed by mutation to TAC. Following replication of the DNA, what change in the polypeptide product would this cause?arrow_forward
- Define and indicate the significance of DNA ligasearrow_forwardWhen circular DNA is sequenced, the nucleotide base pairs are numbered starting from a fixed position on the DNA, all the way around, usually in a clockwise manner. a DNA molecule that is 3133 base pairs long is digested with RsaI restriction enzyme recognition sites at base numbers 366, 1534, and 2207. What are the sizes of the DNA fragments that will be produced after the DNA is digested with RsaI?arrow_forwardThe enzymes DNA polymerase in E.coli is a DNA dependent polymerace and also has the ability to proof-read the DNA strand being synthesised?arrow_forward
- Several temperature-sensitive mutant strains of E. coli displaythe following characteristics. Predict what enzyme or function isbeing affected by each mutation. Supercoiled strands remain after replication, which is never completed.arrow_forwardName the enzymes that cut the DNA at specific sequences and create sticky ends.arrow_forwardIdentify the dinucleotide CA repeat region and the score in the following sequence:TGGCACACTCACACCACACAGACAGTTAarrow_forward
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